Lecture #5 Photosynthesis and light independent

Question 1

How science works

Answer 1

You can not prove something in a hypothesis. You can only prove things are not fact unless you cannot prove it.

Question 2

What do all plants have?

Answer 2

They have a PCR/CBB cycle

Question 3

Calvin-Benson-Bassam-Cycle(stoichiometry)

Answer 3

-One cycle takes in one CO2
-One cycle builds the pools of intermediates
-Three cycles takes in three CO2
-Three cycles balance the input and output
-Three cycles create enough of a pool to output: One G3P (3C’s)
- Six cycles result in enough G3P for glucose to be made (DOES NOT OCCUR IN THE CBB)

Question 4

RubisCO- Ribulose bisphosphate carboxylase/oxygenase

Answer 4

-catalyze RuBP + CO2 -> 2x3PGA
-Most abundant protein on Earth
-40 percent of soluble protein of most leaves
- Very large protein (500,000 amino acid)
-16 subunits, 8 have active sites

Question 5

Calvin Beson-Bassam Cycle C3 plants

Answer 5

Note: Cycle is three times because it can only take in 1 CO2 at a time. 3xCO2 for 1 x G3P = sucrose

Carboxylation:
Intake 3 CO2 + 3 x RuBP (Rubisco)-> 6x 3-PGA
2. Reduction
- 6x ATP used into 6 x 3-PGA -> 6BPG + 6x ADP
- Added 6 X NADPH is oxidized with 6x BPG to -> 1 x G3P (sucrose making) + 5x G3P + 6x (NADP+ + Pi + H+)
3. Regeneration
- Intermediates ( 5 x G3P ) and 3 x ATP to regenrate 3 x RuBP

Question 6

Diffusion

Answer 6

statical tendancy for equalization of different concentrations of molecules, tendency to be random
-Due to random thermal movement of molecules- therefore temperature responsive

Question 7

What is the diffusivity of CO2 across the membrane
and factors that influence diffusivity

Answer 7

1 x10 ^-12
Temp +
molecular weight -
shape(proteins) -
interactions with other molecules