Lecture 5 - Microarrays/DNA Sequencing Flashcards
Cloning Definition
Massive Amplification of DNA/RNA sequences
Microarray Definition
Set of DNA Sequences representing entire genome arranged in a grid for genetic testing
5 types of Arrays
SNP Array
Array CGH
Gene expression profile
Chromatin Immunoprecipitation
Methylation arrays
DNA Sequencing Definition
Determination of DNA sequence of nucleotides
Features of DNA sequences
Individual genomes are unique except for monozygotic twins
~0.1% variation between individuals
Sanger Sequencing Definition
First type of Sequencing
Used in HGP
Various names of Second Gen Sequencing
Next Gen Sequencing (NGS)
Massively Parallel Sequencing
Deep Sequencing
Second Gen Sequencing General Features
Library Dependent
Library fragments amplified on a solid surface
Reactions take place in parallel
Read shorter fragments
2 types of Third Gen Sequencing (TGS)
PacBio SMRT
Oxford Nanopore Technologies
2 types of PacBio SMRT
Continuous Long Read
Circular Consensus Sequencing
Breadth of Coverage Definition
Amount of genome being covered at a certain depth
Expressed as a percentage