Lecture 5 Flashcards

1
Q

What precursors are used to synthesise ACh?

A
  • choline - acetyl coenzyme A
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2
Q

What enzyme synthesise ACh?

A

Choline acetyltransferase (ChAT)

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3
Q

What have amyloid proteins been implicated with?

A

Inhibiting ChAT in patients with Alzheimer’s disease

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4
Q

What is choline uptake blocked by?

A

Hemicholinium 3 (HC-3)

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5
Q

Is hemicholinum 3 competitive or non-competitive

A

Competitive

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6
Q

What is uptake and storage blocked up?

A

Vesamicol

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7
Q

How does tetrodotoxin work?

A
  • blocks voltage gated Na+ channels - inhibits action potentials - no ACh release
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8
Q

How do conatoxins work?

A
  • blocks P/Q and N type voltage gated Na+ channels - inhibits Ca2+ influx - no ACh release
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9
Q

How do dendrotoxins work?

A
  • block voltage-gated K+ channels - prolongs Ca2+ influx - increased ACh release
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10
Q

How does ziconotide work?

A
  • selective N-type voltage-gated Ca2+ channel blocker - given via intrathecal route to manage severe pain
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11
Q

Mechanisms of botulinum toxin action

A

-blocks vesicular release of ACh causing flaccid paralysis - binds to exposed synaptotagmin (Ca2+ sensor) - light chain (a protease) cleaves from heavy chain and attacks SNARE proteins (SNAP-25, syntaxin or synaptobrevin) at a neuromuscular junction - preventing vesicles from anchoring to the membrane to release ACh

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12
Q

What are the effects of botulinum toxin?

A
  • treats dystonias= muscular spasms e.g. belpharospasm (eye muscules) - treats muscle spasticity, tremor - treats sialorrhea (drroling), hyperhidrosis (sweating - cosmetic uses
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13
Q

what do miniature end-plate potentials (MEPP) represent?

A

The release of a single quanta of vesicular content at the NMJ

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14
Q

What does 𝛼-latrotoxin do?

A
  • initial excess ACh release= muscle spasms - afterwards distends nerve terminals - depletion of vesicle pool, desensitisation and inhibition of endocytosis= paralysis
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15
Q

Is the release between synaptic vesicles and quantum of chemical transmitter equivalent or not?

A

Equivalent

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16
Q

How does 𝛼-latrotoxin work?

A
  • dimers= promotes and enhances Ca2+ release - tetramers= embeds itself into channel and is Ca2+ permeable
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17
Q

what do miniature end-plate potentials (MEPP) become?

A

End plate potential (EPP)

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18
Q

What happens if an (EPP) is large enough?

A

Initiate an action potential causing muscular contraction

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19
Q

What enzyme terminates ACh at the NMJ?

A

Acetylcholinesterases (AChE)

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20
Q

What are anticholinesterases?

A

Drugs that inhibit AChE

21
Q

What do anticholinesterases do?

A
  • increase concentration and effects of ACh - can cause muscle spasms
22
Q

What is an example of a competitive non-depolarising blocker?

A

Tubocurarine

23
Q

What happens when you use a competitive blocker with an anticholinesterase?

A
  • anticholinesterase increases the concentration of agonists - regular muscle response returns
24
Q

Is this the action of a competitive or irreversible blocker?

A

Competitive non-depolarising blocker

25
What is an example of an irreversible non-depolarising blocker?
𝛼-bungarotoxin
26
What happens when you use a irreversible blocker with an anticholinesterase?
- no effect - even increasing the agonist concentration cannot outcompete the irreversible blocker
27
Is this the action of a competitive or irreversible blocker?
Irreversible non-depolarising blocker
28
Names examples of competitive non-depolarising blockers
- tubocurarine - vecuronium - rocuronium
29
What are the side effects of tubocurarine?
- decreased BP due to ganglion block - resultant vasodilation - respiratory paralysis
30
Why would you use vecuronium and rocuronium over tubocurarine?
- fewer side effects - rocuronium is rapid in onset
31
What are competitive blockers reversed by?
Anticholinesterases e.g. neostigmine
32
What are the two stages that occur from depolarising blockers ?
- Phase I block - Phase II block
33
What happens in Phase I block?
- persistent activation of endplate nAChRs - prolonged desensitisation of endplate - inactivation of voltage-gated Na+ channels
34
What happens in Phase II block?
- desensitisation of endplate nAChRs - repolarisation of endplate - receptor desesnitisation maintains blockade
35
What is an example of a depolarising blocker?
Suxamethonium
36
Why would you use suxamethonium clinically?
- rapid onset of paralysis - short duration (broken down by plasmacholinesterases) - tracheal intubation - electroconvulsive therapy
37
What are the side effects of suxamethonium?
- bradycardia= M2 mAChR activation - K+ release in trauma (e.g. burns)= cardiac dysrythmias and arrest - prolonged paralysis (1:3500 people)
38
What are the subtypes of ganglionic nicotinic receptors?
- 𝛼 (primarily 𝛼3) - β (primarily β4)
39
What are the effects of ganglionic blockers?
Reduced actions of both the sympathetic and parasympthetic nervous systems
40
What are examples of irreversible ganglionic antagonists?
- 𝜅-bungarotoxin - hexamethonium - tubocurarine
41
What are examples of competitive ganglionic antagonists?
Trimethaphan
42
Why would you use trimethaphan?
Occasionally used in surgery for controlled hypotension and hypertensive crises
43
How does hexamthonium and tubocurarine work at the ganglion?
Drug sits in channel like plug in a bottle
44
What would you use hexamthonium for?
Hypertension
45
What are the side effects of hexamethonium?
- dry mouth and skin - blurred vision - constipation - urinary retention - postural hypotension
46
What are examples of ganglionic agonists?
- nicotine - lobeline
47
How do nicotine and lobeline work at the ganglion?
- repeatedly stimulate receptors - inactivate voltage-gated Na+ channels - desensitise nAChRs
48
Would suxamethonium generate an effect at ganglionic nAChR?
No