lecture 5

Question 1

vesicles at cisterna

Answer 1

• tips fuse or pinch off

Question 1

three types of golgi?

Answer 1

cis, medial, trans

Question 2

COP2 vesciles

Answer 2

RER to Golgi uses these vesicles for anterograde transport

Question 3

COP1 vesicle

Answer 3

cis-Golgi to RER using retrograde transpor

Question 4

clathrin veicles

Answer 4

TGN/PM to late endosomes

Question 5

GTP binding proteins

Answer 5

• control assembly and disassembly of cop 2 coat proteins and docking vesicles to membrane

Question 6

DXE

Answer 6

• used by certain cargo membranee proteins sorting signal that is recognized by cop 2 protein

Question 7

Do golgi sub-compartments differ in the enzymes they contain

Answer 7

• yes they might have glycosidases or glycosyltransgerases

Question 8

Gycosyltransferases

Answer 8

Add sugars

Question 9

Glycosidases

Answer 9

Removing sugars

Question 10

Cis golgi enzymes

Answer 10

Glycosidases

Question 11

Medial golgi enzymes

Answer 11

Transferases and Sudanese

Question 12

Trans folgi

Answer 12

• transferases

Question 13

Where does sugar addition to proteins start

Answer 13

In eR, but continues elsewhere

Question 14

Why are glycosulations important

Answer 14

Important for sorting proteins to the lysosome

Question 15

Trans golgi network

Answer 15

Must decide if a protein si going to be secreted or go to lysosome.

Question 16

Lysosome

Answer 16

Contains acidic hydro lasers that function at low pH that divest problematic material

Question 17

What is the order of golgi sub compartments starting from ER

Answer 17

• cis golgi
• medial golgi
• trans golgi

Question 18

Clathrin

Answer 18

It is a three legged protein

Question 19

How does clathrin cause physical deformation of membrane to make it round

Answer 19

Assembles with other clathrin structures to form a soccer ball structure

Question 20

How are cytotoxic proteins and organelles degraded

Answer 20

• by their enclosure in a double membrane vesicle (an autophagosome) which fuses with lysosomes

Question 21

Autophagosomes

Answer 21

• a double membrane vesicle

Question 22

what types of proteins have KDEL sequences

Answer 22

• luminal proteins including chaperons (e..g, BiP) and lectins (like calreticulin) have KDEL sequences

Question 23

triskelion structure

Answer 23

• heavy chain, light chain, binding site for assembly partilces