Lecture 5

Question 1

Mannitol Salt Agar

Answer 1

A selective medium for the isolation of
presumptive pathogenic staphylococci.

Question 2

Egg-Yolk Agar

Answer 2

For demonstration of lecithinase
activity.

Question 3

Requirements of Neisseria spp culture

Answer 3

1. Good specimen, transport and correct inoculation.
2. Growing small inocula of demanding
   strains.
3. Optimal incubation temperature and
   gaseous environment.
4. Selective agents to prevent overgrowth
   of commensal organisms.

Question 4

Thayer Martin Medium (Modified)

Answer 4

1. Contains a highly nutritive mixture of
   meat and plant enzymatic digests.
2. Starch ensures the absorption of toxic
   metabolites produced by Neisseria spp.
3. Phosphate buffers prevent changes in pH
   due to amine production.

Question 5

Mueller-Hinton Agar

Answer 5

An antimicrobial susceptibility testing
medium.

Question 6

Mueller-Hinton Agar Use

Answer 6

Used in internationally recognized
standard procedures.

Question 7

DNase Agar

Answer 7

Used for the detection of microbial
deoxyribonuclease enzymes.

Question 8

Triple Sugar Iron Agar

Answer 8

Differentiation of Enterobacterales by
three sugar fermentations and H2S
production.