Lecture 1 Flashcards
Define Culture Medium
Formulation of substances in liquid, semi-solid, solid form which contain natural or synthetic constituents intended to support multiplication or to preserve viability of microorganisms
Types of Liquid Media
Storage media and Enrichment media
Types of semi-solid Media
Transport media and OF medium
Types of Solid Media
Basal media, enriched media, selective media, differentiating media
Susceptibility Testing Media
- Support bacterial growth
- Do not inhibit anti-microbial diffusion
- Can be enriched
Blood Culture Media
- Can have anaerobic / fastidious bacteria
- Can have aerobic bacteria
The two common ingredients in culture media are
Water and Agar
Key Ingredients in culture media
- Nutrients
- Energy
- Essential metals + Minerals
- Buffering agents
- Indicators for pH changes
- Selective agents
- Gelling agents
- Other ingredients
Two polypeptides found in agar are
Agarose and Agaropectin
Function of Agarose
High strength gelling properties
Function of Agaropectin
Viscous properties
Types of Agar
- Bacteriological agar
- Processed bacteriological agar
- Technical grade agar
Use of bacteriological agar
Used in culture media
Use of processed bacteriological agar
Used in susceptibility testing media
Use of technical grade agar
Used in immuno-electrophoresis and gel diffusion studies
