Lecture 1 Flashcards
Define Culture Medium
Formulation of substances in liquid, semi-solid, solid form which contain natural or synthetic constituents intended to support multiplication or to preserve viability of microorganisms
Types of Liquid Media
Storage media and Enrichment media
Types of semi-solid Media
Transport media and OF medium
Types of Solid Media
Basal media, enriched media, selective media, differentiating media
Susceptibility Testing Media
- Support bacterial growth
- Do not inhibit anti-microbial diffusion
- Can be enriched
Blood Culture Media
- Can have anaerobic / fastidious bacteria
- Can have aerobic bacteria
The two common ingredients in culture media are
Water and Agar
Key Ingredients in culture media
- Nutrients
- Energy
- Essential metals + Minerals
- Buffering agents
- Indicators for pH changes
- Selective agents
- Gelling agents
- Other ingredients
Two polypeptides found in agar are
Agarose and Agaropectin
Function of Agarose
High strength gelling properties
Function of Agaropectin
Viscous properties
Types of Agar
- Bacteriological agar
- Processed bacteriological agar
- Technical grade agar
Use of bacteriological agar
Used in culture media
Use of processed bacteriological agar
Used in susceptibility testing media
Use of technical grade agar
Used in immuno-electrophoresis and gel diffusion studies
What are peptones
Peptones are water soluble products obtained form lean meat and other protein material. These provide substance to the organism
What are peptones
Peptones are water soluble products obtained form lean meat and other protein material. These provide substance to the organism
Types of Peptones are
- Soya Peptone
2.Casein Hydrolysate
3.Malt extract
Types of Blood and Blood products
- Horse Blood
- Sheep Blood
- Horse Serum
