Lecture 5

Question 1

What does the electron transport chain do?

Answer 1

converts “reducing power” in the form of NADH and FADH2 into direct ATP energy currency

Question 2

where does electron transport chain occur?

Answer 2

inner mitochondrial matrix

Question 3

Which complexes are involved in proton pumping to the intermembrane space?

Answer 3

complex, 3, 1, 4

Question 4

where is complex 2 and what is its role?

Answer 4

sits on the outer surface of the membrane and not involved in proton pumping

Question 5

What is the purpose of a prosthetic group which each complex has

Answer 5

these prosthetic groups fascilitate the transfer of electrons from donor molecules such as NADH to acceptors within the membrane

Question 6

What is the name of complex 1

Answer 6

NADH CoQ oxidoreductase

Question 7

What is the name of complex2

Answer 7

succinate CoQ oxidoreductase

Question 8

What is the name of complex 3

Answer 8

CoQH2 cytochrome c oxidoreductase

Question 9

What is the name of complex 4

Answer 9

cytochrome c oxidase

Question 10

Overall in aerobic respiration how much NADH, ATP and FADH2 are produced?

Answer 10

10 NADH
2 FADH2
4 ATP

Question 11

overall the process of the electron transport chain is what kind of phosphorylation

Answer 11

oxidative phosphorylation

Question 12

in general define chemiosmosis

Answer 12

H+ being pumped back into the mitochondrial matrix via ATP synthase pump

• an energy coupling mechanism that uses energy stored in the form of H+ gradient across a membrane to drive cellular work

Question 13

true or false: cyt c and CoQ are part of the complexes for the electron transport chain?

Answer 13

false; they are not; they are free to move about in the inner membrane; they just connect the complexes as they shuttle the electrons from one complex to the next

Question 14

Is ATP generated during chemiosmosis?

Answer 14

yes, by the movement of hydrogen ions across the membrane during cellular respiration

Question 15

true or false; for every two electrons, one O2 molecule is reduced to two molecules of H2O

Answer 15

true

Question 16

How is the ATP produced via ATP synthase?

Answer 16

The H+ ions which move down the rotor causes it to spin, this causes a conformational change in the knob region, activating catalytic sites where ADP and inorganic phosphate combine to make ATP

Question 17

where is the proton gradient between?

Answer 17

the matrix and the intermembrane space

Question 18

what subunits make up the stator?

Answer 18

a, b, delta

Question 19

What makes up the rotor?

Answer 19

gamma and epilson subunits

Question 20

How much ATP can each NADH produce in the e transport chain?

Answer 20

2.5

Question 21

how much ATP can each FADH2 produce in the e transport chain?

Answer 21

1.5

Question 22

how much ATP is produced in aerobic respiration?

Answer 22

32 ATP

Question 23

how much ATP can a glucose molecule make in oxidative phosphorylation?

Answer 23

28 ATP

Question 24

how much ATP can glucose make in substrate level phosphorylation

Answer 24

4 ATP