Lecture 2 Flashcards

1
Q

How many irreversible glycolytic reactions are there?

A

3

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2
Q

Name the 3 irreversible pathways of glycolysis

A

PFK ( F6P to FBP)
hexokinase (glucose to G6P)
Pyruvate kinase (PEP to pyruvate)

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3
Q

Where does glycolysis occur?

A

cytoplasm

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4
Q

true or false; glycolysis occurs in the absence of oxygen?

A

true

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5
Q

true or false; glycolysis is a necessary first step in aerobic respiration and fermentation

A

true

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6
Q

how much ATP and NADH are produced?

A

2 ATP and 2 NADH for every glucose molecule

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7
Q

what are the 2 phases of glycolysis?

A

energy investment phase and energy generation phase

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8
Q

What does it mean by catabolic reaction?

A

break down large molecule

provide energy for ATP

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9
Q

What does it mean by ANAbolic reaction

A

use small molecule to build large ones

require energy

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10
Q

What is the first law of thermodynamics

A

energy can neither be created nor destroyed although maybe changed from one form to another

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11
Q

What is the second law of thermodynamics

A

the entropy of any closed system if not in thermal equilibrium almost always increases

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12
Q

What does it mean by Gibbs Free energy

A

The energy (G) that can be converted to work at a uniform temperature and pressure throughout a system

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13
Q

What does reducing power mean?

A

refers to the addition of electrons to NAD+ to produce NADH

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14
Q

How many ways does glycolysis produce energy?

A

2

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15
Q

What are the 2 ways that glycolysis produces energy?

A

directly via substrate level phosphorylation

indirectly by the reduction of NAD+ to NADH (NAD+ captures released electrons to produce NADH)

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16
Q

What does Hexokinase catalyse?

A

the first step of the glycolytic pathway

  • glucose to G6P
  • requires the investment of 1 ATP
  • can also phosphorylate galactose, fructose and mannose
17
Q

What can inhibit hexokinase?

A

high levels of G6P (under substrate level control)

18
Q

Describe PFK (phosphofructokinase)

A

tetramer

  • has both catalytic and allosteric sites
  • the effectors (ATP; fructose 2,6 bisphosphate) whether activating or inhibitory; bind to the same pocket (active site)
19
Q

what is the role of PFK

A

commits glucose down the glycolytic pathway

20
Q

what are the stimulators of PFK

A

high AMP

fructose 2,6 bisphosphate (overcomes the inhibitory effects of ATP even at relatively high ATP)

21
Q

What are the inhibitors of PFK

A

high levels of ATP

citrate

22
Q

what is the role of pyruvate kinase

A

conversion of PEP to pyruvate

23
Q

What is pyruvate kinase inhibited by

A

high levels of ATP
acetyl CoA
alanine

24
Q

What is pyruvate kinase stimulated by?

A

F1,6BP

25
Q

How many carbons does pyruvate have?

A

3 carbons

26
Q

What is the difference between F1,6BP and F2,6BP

A

both produces by F6P but by different enzymes