Lecture 4 - The Eukaryotic Cell; The Nervous System Flashcards
Nucleoplasm
aqueous soup inside nucleus
Nucleus
- Major feature distinguishing eukaryotic cells from prokaryotic cells
- contains all DNA -> cannot leave nucleus
- Wrapped in a double phospholipid bilayer called the nuclear envelope or membrane
Nuclear Pores
- RNA leaves nucleus through them
- Large holes that perforate the nuclear envelope
Nucleolus
- Area within the nucleus where rRNA is transcribed and subunits of ribosome are assembled
- Isn’t separated from nucleus by a membrane
Endocytosis
- Way cells can acquire substances from the extracellular environment
- Types: Phagocytosis, pinocytosis, and receptor mediated endocytosis
Phagocytosis
- cell membrane protrudes outward to envelope and engulf particulate matter
- only a few specialized cells are capable of it
- the binding of proteins on the matter to protein receptors on the phagocytotic cell is the force that makes it happen
Phagocytosis in Humans
Antibodies or complement proteins bind to particles & stimulate receptor proteins on macrophages and neutrophils to initiate it
Phagosome
What the membrane bound body is called once the particulate matter is engulfed
Pinocytosis
- Extracellular fluid is engulfed by small invaginations of the cell membrane
- Performed by most cells in a random fashion
- nonselective
Receptor-Mediated Endocytosis
specific uptake of macromolecules such as hormones or nutrients
Exocytosis
opposite of endocytosis
Eukaryotic Membrane
- phospholipid bilayer
- similar to prokaryotes but it invaginates and separates to form individual, membrane-bound organelles and compartments
Endoplasmic Reticulum
- Thick maze of membranous walls
- Separates cytosol from the ER lumen or cisternal space
- In many places it is contiguous with the cell membrane and nuclear membrane
ER Lumen
- AKA cisternal space
- The extracellular fluid side of the ER
- Contiguous in places with the space between the double bilayer of the nuclear envelope
Rough ER
- Synthesizes all proteins not used in cytosol
- AKA granular ER b/c granular appearance
- ER near nucleus that has many ribosomes attached to it on the cytosolic side
Translation on Rough ER
- Propels proteins into the ER lumen as they are created
- The newly synthesized proteins are moved through the lumen toward the golgi.
- Small transport vesicles bud off from the ER and carry the proteins across cytosol to Golgi
Golgi Apparatus
- AKA golgi complex
- Series of flattened, membrane bound sacs
- Organizes and concentrates the proteins as they are shuttled, progressively outward from one compartment to the next
- Modifies and packages proteins for use in other parts of the cell and outside the cell
Cisterna
compartment of golgi
How proteins are distinguished in golgi
based upon signal sequence and carb chains
Bulk Flow
Process that packages proteins not possessing a signal sequence into secretory vesicles and are expelled from cell
How golgi changes proteins
glycosylation or removing amino acids
End-Product of Golgi
vesicle full of proteins
What happens to vesicle full proteins?
- expelled from cell as secretory vesicles
- released from golgi to mature into lysosomes
- transported to other parts of cell such as mitochondria or back to ER
Secretory Vesicles
- AKA zygomen granules
- may contain enzymes, growth factors, or extracellular matrix components
- release their contents through exocytosis
Lysosomes
- contain hydrolytic enzymes that digest substances taken in by endocytosis
- came from golgi
Smooth ER
- site of lipid synthesis including steroids
- helps to detoxify some drugs
Cytosol vs. ER Lumen
- cell can be divided into these 2 parts
- in order to reach cytosol, a substance must cross membrane via passive or facilitated diffusion, or active transport
- it can reach the ER lumen via endocytosis without ever transporting across a membrane
Cytoskeleton
- network of filaments
- determines structure and motility of a cell
- anchors some membrane proteins and other cellular components, move components within cell and moves cell itself
Two Major Types of Filaments in Cytoskeleton
- microtubules and microfilaments
Microtubules
- rigid, hollow tubes made from tubulin
- makes spindle apparatus
- makes flagella and cilia
- larger than microfilaments
- have a positive and negative end
Peroxisomes
- production and break down of hydrogen peroxide
- vesicles in cytosol
- grow by incorporating lipids and proteins from cytosol
- inactivate toxic substances
- regulate oxygen concentration
- play role in synthesis and break down of lipids
- play role in metabolism of nitrogenous bases and carbs
Microfilaments
- squeeze membrane together in phagocytosis and cytokinesis
- contractile force in microvilli and muscle
- produce contracting force in muscle
- active in cytoplasmic streaming, phagocytosis, and microvilli movement
Eukaryotic Flagella & Cilia
- specialized structures
- the axoneme of each contains 9 pairs of microtubules forming a circle around 2 lone microtubules -> 9+2 arrangement
- cross bridges connect each of the outer pairs of microtubules to their neighbor
Axoneme
major portion of flagellum and cilium
Dynein
protein that makes up cross bridges
Cross Bridges
- creates a whip action in cilia causing fluid to move laterally
- creates a wiggle action in flagella causing fluid to move directly away from cell (Prok. flagella rotate)
Centrosome
the major microtubule-organizing center (MTOC) in animal cells
Centrioles
Function in production of flagella and cilia
- End of Microtubule
attaches to MTOC in cell
+ End of Microtubule
grows away from MTOC @ positive end
Actin
Major component of microfilaments
Cellular Junctions
- 3 types that connect animal cells
1. tight junctions
2. desmosomes
3. gap junctions - each performs a different function
Tight Junctions
- Form watertight seal from cell to cell that can block water, ions, and other molecules from moving around and past cells
- Epithelial tissue in organs like the bladder, intestines, and kidneys
- Also acts as a barrier to protein movement between the apical and basolateral surface of a cell
Apical Surface
The part of a cell facing the lumen of a cavity
Basolateral Surface
The opposite side of the apical surface of a cell
Desmosomes
- Join 2 cells at a single point
- Attach directly to cytoskeleton of each cell
- Don’t prevent fluid from circulating around all sides of a cell
- Found in tissues that experience a lot of stress like skin & intestinal epithelium
- Often accompany tight junctions
Gap Junctions
- Small tunnels connecting cells
- Allow small molecules and ions to move between cells
- In cardiac muscle provide for the spread of the action potential from cell to cell
Mitochondria
- powerhouse of eukaryotic cell
- where kreb’s cycle takes place
- have own circular DNA that replicate independently from the eukaryotic cell
- surrounded by 2 phospholipid bilayers
- Have own ribosomes -> 55-60s in humans
Endosymbiont Theory
Mitochondria may have evolved from a symbiotic relationship between ancient prokaryotes and eukaryotes
Mitochondrial DNA
- Contains no histones or nucleosomes
- few dozen to several hundred molecules of DNA in each mitochondria
- genes in it code for mitochondrial RNA that is distinct from the RNA in the rest of the cell
- Most proteins used by mitochondria are coded for by nuclear DNA, not mitochondrial DNA
- Passed maternally
Inner Membrane of Mitochondria
- Invaginates to form cristae
- holds the ETC
Intermembrane Space
between the inner and outer membrane
Extracellular Matrix
- In some tissues, cells called fibroblasts secrete fibrous proteins such as elastin and collagen that form a molecular network that holds tissue cells in place
- Different tissues from different matrices
- Can be large part of tissue (as in bone) or small part
- Consistency may be liquid or solid
- May provide structural support, help to determine the shape & motility, and affect cell growth
Three Classes of Molecules That Make up Animal Cell Matrices
- Glycosaminoglycans & Proteoglycans
- Structural Proteins
- Adhesive Proteins
Basal Lamina
- AKA basement membrane
- Thin sheet of matrix material that separates epithelial cells from support tissue
Four Basic Types of Tissue
- Epithelial
- Muscle
- Connective
- Nervous
Epithelial Tissue
Separates free body surfaces from their surroundings
Connective Tissue
- Extensive Matrix
- Blood, lymph, bone, cartilage, and connective tissue proper making up tendons and ligaments
Intercellular Communication
- So organism can function as a single unit
- Communication accomplished chemically via 3 types of molecules:
1. neurotransmitters
2. local mediators
3. hormones - governed by the nervous system, the paracrine system, and the endocrine system
Major Distinction between Methods of Communication
The distance travelled by the mediator
Neurotransmitters
Travel over very short intercellular gaps
Local Mediators
Immediate area around the cell from which they were released
Hormones
Travel throughout the organism via bloodstream
Neurotransmitter vs. Hormonal Mediated Communication
- Neuronal communication: rapid, direct & specific
- Hormonal communication: slower, spread throughout the body, and affect many cells and tissues in many diff ways
Paracrine System
intermediate communication system