Lecture 4 - Study Guide

Question 1

proteins can be purified base on ____, ___ (using ___ ___), or ___ ____

Answer 1

size, pI (using 2D gels), or affinity chromatography

Question 2

protein ____ can be assessed using SDS PAGE gels

Answer 2

purity can be assessed using SDS PAGE gels

Question 3

___ ___ calculations are used to model ___ ___

Answer 3

molecular mechanics calculations are used to model protein structures

Question 4

__-___ ____ and ___ ____ are used to determine ___ ____ structures of ____

Answer 4

x-ray crystallography and 2D NMR are used to determine high resolution structures of proteins

Question 5

__-____ ____ identified in protein structures can be tested by ___ ___

Answer 5

non-covalent interactions identified in protein structures can be tested by mutant analysis

Question 6

___, ___, and ___ ___ can be used to measure ___ ___ to generate ___ ___ ___

Answer 6

UV/Vis, CD, and fluorescence spectroscopy can be used to measure protein folding to generate van’t Hoff plots

Question 7

____ and ___ spectroscopy can be used to monitor ___ and ___ ____ at different ____ (with ___ for fast reactions)

Answer 7

chromatography and UV/Vis spectroscopy can be used to monitor reactant and substrate concentrations at different timepoints (with quenching for fast reactions)

Question 8

___ of ___ or ___ to ___ can be used to test ____ and ___ mechanisms

Answer 8

mutations of enzymes or changes to reactants can be used to test binding and catalysis mechanisms

Question 9

___/___ and ___/____ can be substrates as well as ____ can be, so their characterization techniques can be used to ___ ___ (e.g. observing ___ ___ on a ___)

Answer 9

DNA/RNA and peptides/proteins can be substrates as well as enzymes, so their characterization techniques can be used to monitor reactions (e.g. observing DNA cleavage on a gel)