Lecture 4 Cytoskelton II Flashcards
What associated Proteins are involved in:
-Actin Nucleation
-MT Nucleation
What effect of Nucleation proteins on lag phase
ACTIN: uses ARPs to help nucleation it attaches at minus end and causes elongation in the plus end
MT: uses Gamma TUbulin to promote nucleation binds at the minus end and stabilizes the polymer
This helps get passes the lag phase to go straight into the elongation phase
What cell structures are formed by:
Actin
MT
Actin: helps w/ cell signaling
MT Helps w/ structure
Describe the function roles of MT associated MAPs
MT associated proteins bind to the sides to stabilizer
Describe why both growth AND shrinkage rates are slower when actin filaments are capped w/ CAPz
When CAPZ is present it stabilizes the plus end so when there is depolymerization then its only at minus end
but for other molecule there is depolymerization at both plus and minus end.
Formin
Nucleates assembly and remains associated w/ growing plus end
Actin
Cofliin
Binds ADP actin filaments accelerates disassembly
Actin helps w/ cell mobility
Fimbrin
stress fibers and are short
structural only in actin
A-Actin
helps w/ contractibility
long so they can help w/ contraction
actin only
Spectrin
Provides red blood cell shape and elasticity
actin only
Capping protein
Prevents assembly and disassembly at the plus end
actin
Profillin
Binds subunits and specs elongation in actin subunits
promotes elongation when Cc is less than CcT
ARP complexes
Nucleates assembly to from a web and remains associated at minus end
Actin
gamma Turc
ABTUbulin
Nucleates assembly remains associated at minus end
MT
Kinesin-13
Induces catastrophe and disassembly
MT
TAU
MT
stabilized MT
MAPS
Stabilize MT by binding along sides
XMAPS215
Stabilizes plus end and accelerates assembly
still adding on plus end
Dyenins go to which side
minus end
