Lecture 4

Question 1

Rational protein design is the same as directed evolution

Answer 1

False. Irrational protein design is the same as directed evolution

Question 2

The two types of irrational protein design are error prone PCR and DNA shuffling

Answer 2

True

Question 3

The normal function of Taq polymerase is adding nucleotides to the growing strand and proof reading

Answer 3

True

Question 4

Having no ddATP in PCR has no effect.

Answer 4

False. It causes in incorrect base to be put in instead

Question 5

Error prone PCR is also known as PCR with high fidelity

Answer 5

False. It is known as PCR with low fidelity

Question 6

Homologous genes are derived from the same ancestors

Answer 6

True

Question 7

Dnase has high specificity

Answer 7

False. It has low specificity

Question 8

Restriction enzymes are specific

Answer 8

True

Question 9

Overhanging primers are a starting point for PCR

Answer 9

True

Question 10

RFLP uses homologous genes

Answer 10

True

Question 11

Primerless PCR is a component of gene shuffling

Answer 11

True

Question 12

DNase I produces smaller fragments than restriction enzymes

Answer 12

False. Restriction enzymes creates smaller fragments than DNase I