Lecture 4

Question 1

what enzyme does REDOX reactions use in metabolic pathways?

Answer 1

DEHYDROGENASES

Question 2

how is glucose trapped and manipulated/oxidised into cells/

Answer 2

glucose transporter recognises glucose and phosphorylates it so it can’t leave (phosphate is charged) - is unrecognisable by glucose transporter as glucose’s shape changes

Question 3

when trapped in cells, what pathway (catabolic/anabolic) is glucose fully committed to?
and what effect does this have on it’s role in metabolism and it’s usage in other pathways?

Answer 3

• catabolism
• passed down through metabolism
• can’t be used in other pathways

Question 4

in glycolysis, what is the net investment and harvest of ATP?
and how much NADH is gained net?

Answer 4

2 ATP invested
4 ATP harvested
2 NADH gained

Question 5

co-factor NAD/NADH+ roles (2)

Answer 5

• used for oxidation in glycolysis
• captures electrons and passes them on

Question 6

substrate level phosphorylation defintion

Answer 6

metabolism reaction
ADP or GDP phosphorylated to ATP or GTP

Question 7

what are the two limiting features of glycolysis?

Answer 7

oxygen and NAD+

Question 8

how many stages/steps are in glycolysis?

Answer 8

10

Question 9

how many phases in glycolysis? and what are their names?

Answer 9

2
energy investment phase
energy pay off phase