Lecture 39 - Stem Cell Engineering

Question 1

What is the source of embryonic stem cells?

L39 S6-7

Answer 1

-inner cell mass of blastocyst

Question 2

How can embryonic stem cells be induced to differentiate into different types of cells?

L39 S10

Answer 2

-exposure to a certain combination/order of different growth factors or substances

Question 3

What complicates the use of embryonic stem cells in organisms that are in a later stage of development?

L39 S12

Answer 3

• lack appropriate cues for differentiation

- often from teratomas that have no axis of formation

Question 4

What is SCeNT?

L39 S16

Answer 4

• removal of nucleus form somatic cell of a patient and injection into oocyte of a donor
• oocyte form blastocyst from which embryonic stem cells are isolated

Question 5

What genes when injected into a fibroblast can induce it to have embryonic stem cell like traits?

L39 S20

Answer 5

• Oct4
• Sox2
• Klf4
• Myc

Question 6

What is iPSC?

L39 S21

Answer 6

Induced pluripotent stem cells

-reprogramming of somatic cells to express genes related to stem cell characteristics