Lecture 30 - Emerging Roles for RNAs

Question 1

Why might a Western blot indicate that a protein is going away while the mRNA for that protein seems relatively stable in the Northern blot?

Answer 1

This is most likey a case of specific translational control. The protein is regulated during or after its synthesis,

Question 2

What happens within ferritin mRNA when intracellular iron is low?

Answer 2

IRE-BP takes on an active conformation and interacts with the IREs in the 5’ end of the ferritin mRNA. That binding is tight enough to block the translation of the ferritin mRNA, presumably by impeding the progress of eIF4A.

Question 3

What is Nanos?

Answer 3

Nanoa is an RNA-binding protein that interacts withthe 3’ UTR of a hunchback droposhile night

Question 4

What is the main we regulated ;roteins post-translationally?

Answer 4

Phosphorylation

Question 5

What post-translational modification will change the electrochemical properties of the protein and acts in trans by degrading it and bringing it to another place?

Answer 5

Phosphorylation