Lecture 3 Safety in the Clinical Lab Flashcards
Routine practice includes
- hand hygiene
- working surface disinfection
- use of PPE
- administrative controls
- environmental controls
- risk assessment
older names for routine practices
standard precautions, universal precautions
PPE is used for
infection control
disinfection
destroys many or all pathogenic microorganism. does not kill sports. means of decontamination
what is presept
sodium dichlorosocyanurate
what is phenokil
phenolic based germicide
decontamination
achieved through autoclave, chemical disinfection and inception. kills microorganism
diff btw disinfection and decontamination
disinfect things that might be contaminated. decontaminate things known to be contaminated
sterilization
kills all bacteria and spores. use of autoclave
autoclave parameters
121 Celcius, 15 psi, 30-60 mins
biological risk groups is about
the organism
biological containment level is about
the environment in which you work with the org
Risk group 1
low individual risk and low community risk
risk group 2
moderate individual risk and low community risk
risk group 3
high individual risk and low community risk
risk group 4
high individual and high individual risk
e.g. of risk group 1
normal flora, lactobacillus
e.g. of risk group 2
clostridium, hepatitis, influenza strains
e.g. of risk group 3
HIV, TB
e.g. of risk group 4
ebola virus, variola virus
physical requirements for level 1 biological contamination
open bench top, use of safe practices and PPE
physical requirements for level 2 biological contamination
BSC, PPE, autoclaves, use of dafe practices and appropriate PPE. most clinical labs
physical requirements for level 3 biological contamination
access control, HEPA filtered exhaust, respiratory protection, safe practices, PPE
physical requirements for level 4 biological contamination
fully seals, worked wear +ive pressure suits, class III BSC, decontamination of exhaust and wastes
BSC classification is based on
cabinet’s protection for the workers, product and environment
what determines the classification of BSC
roomair, contaminated air and filtered air flow patterns
list the classes and types for BSC
Class I
Class II Type A1, Class II Type A2
Class II Type B1, Class II Type B2
Class III
Describe HEPA filters
removed 99.99% airborne particles 0.3 um or greater. delicate construction. tested and certified for use in clinical labs
Describe Class 1 BSC
uncirculated air flows away from operator passes through HEPA & is discharged into atmosphere. operator protection. no product protection
Describe Class II BSC
most common. for working with microorganism in containment L 2,3,4 lab. other personal, product and environment protection.
how care Class II Type A (1and 2) and B (1 and 2) differentiated
by their construction, airflow velocity, airflow pattern and exhaust system
describe Class II type A1
cabinet air is either recirculated into lab or ducted out building by thimble connection after passing HEPA. not suitable for volatile chemicals or radionuclides. avg face velocity 0.38m/s. may have +ive pressure contaminated ducts
describe Class II type A2
cabinet air is either recirculated into lab or ducted out building by thimble connection after passing HEPA. suitable for low levels volatile toxic chemicals and radionuclides. avg face velocity of 0.5 mms. ducts and plenums under negative pressure
Describe Class II, Type B1
hard ducted. Exhaust passes through HEPA to atmosphere. suitable for low levels volatile chemical or radionuclides. contains -ive pressure plenums. min face velocity 0.5 m/s
Describe Class II, Type B2
hard ducted. exhausts 100% of cabinet air thru HEPA to atmosphere. contains -ive pressure plenums. face velocity 0.5m/s
Describe Class III
full air tight enclosure. HEPA filtered supply and exhaust air. cabinet is negative pressure work performed thru long sleeve gloves. protection for worker and product. good for group 4 organisms
