Lecture 3 Safety in the Clinical Lab Flashcards

1
Q

Routine practice includes

A
  • hand hygiene
  • working surface disinfection
  • use of PPE
  • administrative controls
  • environmental controls
  • risk assessment
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2
Q

older names for routine practices

A

standard precautions, universal precautions

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3
Q

PPE is used for

A

infection control

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4
Q

disinfection

A

destroys many or all pathogenic microorganism. does not kill sports. means of decontamination

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5
Q

what is presept

A

sodium dichlorosocyanurate

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6
Q

what is phenokil

A

phenolic based germicide

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7
Q

decontamination

A

achieved through autoclave, chemical disinfection and inception. kills microorganism

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8
Q

diff btw disinfection and decontamination

A

disinfect things that might be contaminated. decontaminate things known to be contaminated

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9
Q

sterilization

A

kills all bacteria and spores. use of autoclave

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10
Q

autoclave parameters

A

121 Celcius, 15 psi, 30-60 mins

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11
Q

biological risk groups is about

A

the organism

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12
Q

biological containment level is about

A

the environment in which you work with the org

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13
Q

Risk group 1

A

low individual risk and low community risk

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14
Q

risk group 2

A

moderate individual risk and low community risk

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15
Q

risk group 3

A

high individual risk and low community risk

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16
Q

risk group 4

A

high individual and high individual risk

17
Q

e.g. of risk group 1

A

normal flora, lactobacillus

18
Q

e.g. of risk group 2

A

clostridium, hepatitis, influenza strains

19
Q

e.g. of risk group 3

A

HIV, TB

20
Q

e.g. of risk group 4

A

ebola virus, variola virus

21
Q

physical requirements for level 1 biological contamination

A

open bench top, use of safe practices and PPE

22
Q

physical requirements for level 2 biological contamination

A

BSC, PPE, autoclaves, use of dafe practices and appropriate PPE. most clinical labs

23
Q

physical requirements for level 3 biological contamination

A

access control, HEPA filtered exhaust, respiratory protection, safe practices, PPE

24
Q

physical requirements for level 4 biological contamination

A

fully seals, worked wear +ive pressure suits, class III BSC, decontamination of exhaust and wastes

25
Q

BSC classification is based on

A

cabinet’s protection for the workers, product and environment

26
Q

what determines the classification of BSC

A

roomair, contaminated air and filtered air flow patterns

27
Q

list the classes and types for BSC

A

Class I
Class II Type A1, Class II Type A2
Class II Type B1, Class II Type B2
Class III

28
Q

Describe HEPA filters

A

removed 99.99% airborne particles 0.3 um or greater. delicate construction. tested and certified for use in clinical labs

29
Q

Describe Class 1 BSC

A

uncirculated air flows away from operator passes through HEPA & is discharged into atmosphere. operator protection. no product protection

30
Q

Describe Class II BSC

A

most common. for working with microorganism in containment L 2,3,4 lab. other personal, product and environment protection.

31
Q

how care Class II Type A (1and 2) and B (1 and 2) differentiated

A

by their construction, airflow velocity, airflow pattern and exhaust system

32
Q

describe Class II type A1

A

cabinet air is either recirculated into lab or ducted out building by thimble connection after passing HEPA. not suitable for volatile chemicals or radionuclides. avg face velocity 0.38m/s. may have +ive pressure contaminated ducts

33
Q

describe Class II type A2

A

cabinet air is either recirculated into lab or ducted out building by thimble connection after passing HEPA. suitable for low levels volatile toxic chemicals and radionuclides. avg face velocity of 0.5 mms. ducts and plenums under negative pressure

34
Q

Describe Class II, Type B1

A

hard ducted. Exhaust passes through HEPA to atmosphere. suitable for low levels volatile chemical or radionuclides. contains -ive pressure plenums. min face velocity 0.5 m/s

35
Q

Describe Class II, Type B2

A

hard ducted. exhausts 100% of cabinet air thru HEPA to atmosphere. contains -ive pressure plenums. face velocity 0.5m/s

36
Q

Describe Class III

A

full air tight enclosure. HEPA filtered supply and exhaust air. cabinet is negative pressure work performed thru long sleeve gloves. protection for worker and product. good for group 4 organisms