Lecture 3: Intro to Histo

Question 1

What type of Microscopy is this?

Answer 1

Light Microscope(LM)
▪Uses photons ▪Highlights gross &
biochemical structure

Question 2

What type of Microscopy is this?

Answer 2

Scanning electron microscopy (SEM) ▪Use electrons ▪Highlights surface
structure

Question 3

What type of Microscopy is this?

RED VS BLUE

Answer 3

Question 4

What components are being stained

Answer 4

Basophilic
components with net neg charge, DNA, RNA, and GAGs

Question 5

What components are stained in the circle

Answer 5

Acidophillic
Actin, myosin, collagen, membranes

Question 6

What stain did this? And why is this a bad image

Answer 6

H&E
Too many pinks need a counterstain such as Trichrome

Question 7

What stain did this? And what is its DX importance

Answer 7

Used to distinguish collagen from muscle & epithelium
Diagnose scar tissue (e.g., liver cirrhosis) and smooth muscle tumors

Question 8

What stain did this, what is it looking for

Answer 8

PAS, Carbs(Glycogen and mucin)

Question 9

What stain did this?

Answer 9

Osmic Acid OR Sudan Red

Looking for Lipids, Adipocytes

Lipids do not survive staining process need to highlight them

Question 10

Which stain did this?

Answer 10

Silver
Looking for Reticular fiber, looks like split ends

Question 11

Which Stain did this?

Answer 11

Orcein

Elastic fibers