Lecture 3: Genes and Transcription

Question 1

RNA Polymerase 1

Answer 1

Transcribes rRNA genes

Question 2

RNA Polymerase 2

Answer 2

Transcribes mRNA/protein coding genes

Question 3

RNA Polymerase 3

Answer 3

Transcribes tRNA

Question 4

5 Transcription Factors for RNA Polymerase 2

Answer 4

Order: TFIID, TFIIB, (RNA Poly 2), TFIIF, TFIIE, TFIIH

DAB Paul Twice For Efficient Healing

Question 5

TFIID

Answer 5

Recognizes TATA box with TBD subunit and and starts the process of transcription.

Question 6

TFIIB

Answer 6

Positions the RNA Polymerase. Second ste.

Question 7

TFIIF

Answer 7

Stabilizes RNA Pol and attracts TFIIE and TFIIH

Question 8

TFIIE

Answer 8

Recruits TFIIH

Question 9

TFIIH

Answer 9

Unwinds DNA at transcript start point. Phosphorylates Ser5 of RNA pol CTD (carboxyterminus domain), releases RNA pol from promoter

Question 10

Enzymes used for creating 5’ cap

Answer 10

Phosphatase=removes phosphate from 5’ end
Guanotransferase= adds gmp in reverse linkage
Methyl transferase= adds methyl group to guanosine