Lecture 3: Enzyme kinetics

Question 1

kinetics

Answer 1

studies the rate of reaction

Question 2

presteady state

Answer 2

• state during the course of a reaction where enzyme substrate is forming

Question 3

First order kinetics

Answer 3

• reaction velocity is directly proportional to subrtate concentration
• substrate conc is low and most enzyme molecules are free to combine with substrate

Question 4

mixed order kinetics

Answer 4

• half enzyme molecules are bound to substrate
• velocity is not proportional to substrate conc

Question 5

zero order kinetics

Answer 5

• all enzymes molecules are bound to substrate
• velocity will remain unchanged because free enzym to bind with excess substrate is not available

Question 6

Assumption of Menten quations

Answer 6

1. there is no product at start of kinetic analysis
2. during reaction equilibrium position is established where rate of formation of ES= rate of breakdown of ES
3. substrate concetration is always greater than enzyme concentration since enzyme can be recycled
4. only the initial velocity is measure hence reverse E+P reaction can be ignored
5. enzyme is either present as a free enzym or bound to the substrate