Lecture 3 Bacterial Genomics

Question 1

What criteria are used to predict functional ORFs?

Question 2

In genome annotation, how are functions assigned to predicted ORFs?

Question 3

How does bacterial genome organization differ from eukaryotic genome organization?

Question 4

How would you analyze the complete transcriptome of a bacterium? What do you learn?

Question 5

Are all genes expresses at all times?

Answer 5

No, not all genes are expressed at all times.

Question 6

What is next generation sequencing?

_____ sequence reads from the DNA sample.
It is very high in _______
Computer algorithms finds ______ between the _____ sequence reads.
Contig are a _______

Answer 6

Short, accuracy, overlaps, short, overlapping,
overlapping DNA sequence

Question 7

____ technology that generates longer reads (____-____ kb) that help to connect contigs to each other. This step is called _____ _____.

Answer 7

Pacbio, 10-15 kb, gap closure