Lecture 3 and 4: Propagation by Cuttings Flashcards
Benefits of cuttings
- more efficient use of space
- least expensive way of cloning plants
- more uniform crop
Topophysis
the position on the stock plant impacts the growth orientation of the propagule
Dedifferentiation
the ability of previously developed, differentiated cells to initiate cell division and form new meristematic regions
Necrotic plate
protective barrier from drying and from potential invading pathogens
callus
hard formation of tissue
4 Stages of Adventitious Root Formation
- Dedifferentiation of cells
- Formation of root initials
- Development of root initials into organized root primordia
- Growth and emergence of the root primordia outward through stem tisssue
What time of year should the cutting be taken?
When a bud begins growth in the spring the shoot is immature and there is very little secondary growth
Deciduous plant are most often propagated by…
softwood cuttings
Broad-leaved evergreems usually root best as…
semi hardwood cuttings
Conifers root best as…
hardwood cuttings
Softwood stage of growth
from bud break until about the time the shoot stops elongating
Semi-hardwood stage of growth
After shoot growth stops, secondary development continues and by early-fall, the plant is preparing for dormancy
Hardwood stage of growth
once dormancy sets in, the shoots are in the hardwood stage of growth
Transpiration
the process that drives water movement in a plant from roots to shoots
How can water loss be managed?
- Mist systems
- Fog
- Low temp
How does mist manage water loss?
mist settles out of the air onto the cuttings and the media which limits transpiration
How does fog manage water loss?
fog reduces the oppurtunities for evaporative cooling
Water logging
can be caused by using a media whose texture is too fine or by over misting
Auxins
- the only plant hormone/PGR that reliably induce rooting
- active ingredient in most rooting compounds in which cuttings are dipped during vegetative production
Indoleacetic Acid(IAA)
- naturally created in leaves and moved down stem to roots
- limited use for rooting since plants and microbes easily break it down
Indole butyric acid(IBA)
- mimic auxin
- can be applied in a liquid or power form
- has to dissolve and then carried into cutting
Naphthaleneaetic acid
-most commonly used PGR after IBA
-Dip n Grow
Etoliation
buds break dormancy and grow in the dark
Phototropism
bending toward a light source
Geotropism
downward root growth in response to gravity
Gibberellins
- stimulates cell division and elongation
- involved in seed dormancy and rate of germination
- inhibits bud, shoot, and root formation
Cytokinins
- stimulates and plays a regulatory roll in cell division
- included in sterile media when growing plants in tissue culture to initiate shoot formation
Ethylene
1.only found in gaseous form
2. governs the development of laves, flowers, and fruits
3. induces ripening
High cytokinins
low auxin ratio stimulates adventitious bud formation and overcomes apical dominance
Abscisic acid(ABA)
- regulates stomata closure
- induces germination
Epinasty
when ethylene damage have leaves that are curled down and stems that are twisted
1-MCP
is a gaseous ethylene action inhibitor