Lecture 3

Question 1

How many electrons do cytochromes carry?

Answer 1

One electron

Question 2

How many electrons do FeS proteins carry?

Answer 2

One electron

Question 3

How many electrons does CoQ carry?

Answer 3

1 or 2 electrons/protons

Question 4

How many electrons do FMN and FAD carry?

Answer 4

One or two electrons/protons

Question 5

What are the layers of the mitochondria in order from outside to inside?

Answer 5

Outer membrane
IMS
Inner membrane
Matrix

Question 6

Where are the complexes located in the mitochondria?

Answer 6

Inner membrane

Question 7

How do we determine the order of the complexes of the electron transport chain?

Answer 7

1. Order the reduction potentials from most negative to most positive
2. Add inhibitors/run spectrophotometry
3. Deprive of O2, then add back in, measure rate of electron flow through each complex

Question 8

What are the 3 enzymes that attenuate ROS?

Answer 8

Superoxide dismutase
Glutathione peroxidase
Glutathione reductase

Question 9

What does superoxide dismutase do?

Answer 9

It reduces the superoxide radical to hydrogen peroxide and molecular oxygen

Question 10

What does glutathione peroxidase do?

Answer 10

It converts hydrogen peroxide into water

Question 11

What does glutathione reductase do?

Answer 11

It recycles glutathione peroxidase back to its reduced form