Lecture 3 Flashcards
1
Q
What is ‘Bivalent’ chromatin state
A
They are the regulatory factors that contains both activating and repressive histone modification which thus causing the differentiation genes be both silenced and ‘poised’ for activation.
2
Q
Does mouse ESCs have fixed state in terms of repressed state or activated state
A
Mouse ESCs in conventional culture are
in a constant state of flux (bivalent state)
3
Q
Is Stem cells prime state and naive state reversible?
A
In a stem cell – both states are reversible.
4
Q
What does LIF/LIFR-gp130/Jak-Stat3 pathway do
A
Klf4, c-myc, Tfcp21/self-renewal
5
Q
What does FGF/FGFR/MEK/ERK pathway do
A
differentiation/inhibit self-renewal
6
Q
What does Wnt/Frizzled-Lrp5-6/β-catenin (GSK3) pathway do
A
Klf4, c-myc
