Lecture 3

Question 1

Pepsin works at ?

Answer 1

PH 2 Stomach

Question 2

Amylase works at ?

Answer 2

PH 7 Mouth

Question 3

Arginase “

Answer 3

PH 10 kidneys

Question 4

As temperature increases what happens to the flexibility in the protein back bone

Answer 4

increases

Question 5

Optimum temperature is dependent on?

Answer 5

hydroponic and hydrophilic interactions

Question 6

K=

Answer 6

turnover number

Question 7

e+s> es

k for forward and reverse reaction

Answer 7

k1 k-1

Question 8

Es>E+ P

K for forward and reverse reaction

Answer 8

k2

not reversible

Question 9

competitive Inhibitor?

Answer 9

has a similar shape to substate and occupies the active site

Question 10

Inhibitor effect is reduced by

Answer 10

substrate concentration

Question 11

Can enzyme controlled reaction reach the same Vmax with and without the inhibitor present?

Answer 11

yes if substate concentration is great enough

Question 12

Km of the inhibited enzyme controlled reaction?

Answer 12

greater than without the inhibitor

Question 13

In linnear regression km?

Answer 13

is closer to zero

Question 14

Non-competitive inhibitor?

Answer 14

a small molecule which binds to a remote site on an enzyme and effects Kcat

Question 15

Where do non competitive inhibitors bind?

Answer 15

allosteric sites

Question 16

effect on active site (when in the presence of a non-competitive inhibitor)

Answer 16

conformational change of the enzyme active site, cannot bind to a substrate

Question 17

Effect non-competitive inhibitor has on km

Answer 17

no effect

Question 18

Y intersect on the liner regression on Micheal-mentons graph when enzyme is in the presence of a non-competitive inhibitor ?

Answer 18

greater

Question 19

Positive regulator ?

Answer 19

activator

Question 20

negative regulator

Answer 20

repressor

Question 21

Uncompetitive inhibitor?

Answer 21

A substrate which binds to the enzyme substrate complex and stops the formation of any further products

Question 22

uncompetitive inhibitors effect what which Is measured on a Micheal menton’s graph

Answer 22

Both Km and Vmax

Question 23

Allosteric inhibition?

Answer 23

In which a product from an enzyme cascade inhibits the first enzyme

Question 24

Curve to represent allosteric kinetics ?

Answer 24

sigmoidal curve not Micheal menton

Question 25

Cleavage activation is an example of?

Answer 25

Covalent regulation

Question 26

Cell signalling an example of?

Answer 26

Covalent regulation

Question 27

Adenylation is ?

Answer 27

addition of AMP to an enzyme

Question 28

Trysin enzyme Is made in?

why?

Answer 28

an inactive form: Tripsogen which when activated by the absence of an inhibitor, becomes trypsin

This mechanism is to stop trypsin damaging cells until outside cells where it is activated

Question 29

Oxidoreducases ?

Answer 29

oxidation reduction reaction

Question 30

Transferases?

Answer 30

transfer chemical groups

Question 31

Hydrolase’s ?

Answer 31

Induce hydrolytic cleavage

Question 32

Lyases

Answer 32

Cleave c-n c-o c=0 by eliminating molecules. Leaving double bonds, rings or conversely addition of groups to double bonds

Question 33

Isomerases

Answer 33

catalyse geometric/strctural changes within molecule

Question 34

Ligases

Answer 34

joining together of two groups coupled with hydrolysis of diphosphate bond