Lecture 1 Flashcards
what kind of conditions do enzymes operate under?
MILD
pH and temp
Positional specificity?
position of a functional group within a molecule will impact its effect on an enzyme
Catalysts effect on equilibrium?
no effect
Mechanism in which catalysts operate by?
chemo-adsorb reactants onto the surface of a reaction of an enzyme, catalyse the reaction, chemo-deadsorb and release the product (rearranged reactants)
Kcat?
turnover number of a catalysed reaction (per unit time)
Kuncat
turnover number of an uncatalysed reaction
Equation for chemical catalysis
Kcat/kuncat
What type of active site do enzymes have?
internal and asymmetric
Two factors effecting whether a reaction will proceed?
delta g (Gibbs free energy) and its activation energy
Gas law? Units
Pv=nrt p= pascals v=m^3 n=moles r= gas constant (given) t= kelvin
Delta delta g++(transition state)?
the different between the activation energy (transition state) of the catalysed vs uncatalysed reaction
Define transition state?
the energy in which the intermediate will enquire at its maximum to react
K depends on what ? Therefore, the reaction to support this?
activation energy, exponentially
4 reasons why enzymes lower Ea required for a reaction?
- Microenviroment is better suited for the reaction to occur
- pressure on bonds within a substrate
- keeps the substrates in the correct orientation
- stabilises the transition state
Three models for substrates binding to enzymes?
induced fit
lock and key
transition state stabilisation
