Lecture 3

Question 1

why sulfide (H2S) Present in water of microbial origin?

Answer 1

Because of detection of SRB with the gas

Question 2

Example of SRP?

Answer 2

Desulfovibrio vulgaris, thermotoga

Question 3

prokaryotic can’t thrive in deep subsurface oil reservoirs with extreme temperature, pressure, salinity ( T/F )?

Answer 3

F, can

Question 4

thermotoga can growth in?

Answer 4

121c° , survive for 2 hours at 130c°

Question 5

why bacteria can’t live at 130c°?

Answer 5

1. Thermal instability of biological molecules
2. Failure of cell repair mechanism

Question 6

thermotoga can growth in?

Answer 6

121c° , survive for 2 hours at 130c°

Question 7

Incpoculated in culture medium Incubated at 30,60,90 to allow growth of diff bacteria

Answer 7

Cultural methods

Question 8

DNA separated from samples for 16srRNA Sequence analysis

Answer 8

non Cultural method

Question 9

• Cell wrapped in sheath like outer membrane (toga)
• Gram ( - )
• Anaerobe
• Like archaeal, give it’s thermophilic adaptation
Which bacteria?

Answer 9

thermotoga

Question 10

• Suitable for bacteria in diluted samples
• Easier and faster result
• no need for confirmation
  Which technique?

Answer 10

MPN Technique

Question 11

Factors affecting SRB?

Answer 11

Effect of temperature
Effect of salinity
Effect of substrate