Lecture 3 Flashcards
In glycolysis, how many ATP are made
2 ATP bc use 2 and make 4
what is the end product of glycolysis
2 pyruvates
how many substrate level phosphorylations occur during glycolysis
2
explain sub level phosph
an enzyme transfers a phosphate from substrate (PEP) to ADP to form ATP and product
what are the componetns of NAD+ and what can it be called
nicotinamide
ribose sugar
P on the 5th C of ribose
can be called a coenzyme or cofactor
NAD+ is a __ _____ ______ agent and is _____ to NADH
2 electrons oxidizing agent and is reduced to
______ is an electron and hydrogen ion transporting molecule
NADH
in a couple redox rx what can the reactant be
an intermediary of glycolysis and it can give off 2 H+
what 2 H+ does the reactant give in a coupled redox rx (NAD+)
a H: hydride (2 electrons on the side)
and a H+ proton that is carried along with the reactant
when a substance is reduced does it loose or gain an electron
it gained an electron
what happens to glycolysis if the NAD wasn’t in the cytoplasm
it will stop glycolysis and will give 2 electrons and H+ to pyruvate so it can form lactate
what is the diff btw pyruvic acid and pyruvate
pyruvic acid is the H+ of pyruvate so without it pyruvic acid is only pyruvate
in the conversion of pyruvate to lactate which is in an oxidized state and which is in a reduced state
pyruvate is oxidized and lactate is reduced
what is the role of phosphofructokinase
rate limiting enzyme for glycolysis
what activates PFK
high levels of ADP and AMP
