Lecture 26 Flashcards
What can we do by comparing genes that are essential in different conditions?
By comparing genes that are essential in two different conditions we can determine the genes which we should target to destroy or aid bacteria which grow in those particular conditions.
What is Caulobacter crescentus?
Caulobacter crescentus is a bacteria which can be in a swarmer state in which it swims around in its aquatic environment, these will permanently attach to specific environments, becoming stalked cells which can then produce new swarmer cells which will find new places to become stalked cells.
What can PCR reactions do to allow us to determine transposon location?
By carrying out a PCR reaction we can create primers which bind specifically to known transposon sequences, by reading on from these we can determine where the transposon inserted.
What occurs for transposon insertion based on gene size? What are the three types of genes with regards to this?
Longer genes have more potential places for entry of transposons, essential genes should have no insertions (or very few, only at the ends) regardless of size. High-fitness genes will have some mutations, this is because they help with survival heavily but are not strictly essential. Some mutations in the middle of essential genes may occur, but this is due to dead cells still leaking some DNA.
What is an fts gene?
fts stands for filamentous temperature sensitive, it is an essential gene.
