Lecture 25

Question 1

What are natural mutants

Answer 1

where genetic change alters the phenotype gives clue to function of disease

Question 2

What is functional molecular genetics

Answer 2

targeting genes to find function
remove
insert
screen

Question 3

What is transgenesis

Answer 3

Engineering as multicellular organism by adding in foreign DNA

The DNA code is universal, so any DNA can be used - synthetic DNA

Question 4

What is CRISPR

Answer 4

clustered regularly interspaced short palindromic repeats

Question 5

What is Cas 9

Answer 5

CRISPR associated protein

Question 6

What is the process of a targeted mutation

Answer 6

a guide RNA is mixed with a Cas 9 to form a complex that is virally inserted into the target organism
Cas 9 enters the nucleus and finds the guide RNA match target sequence
breaks double stranded break in DNA at targer side
repair template allows gene editing

Question 7

What is somatic gene therapy

Answer 7

the transfer of DNA containing functional copies of genes into somatic cells to cure genetic disorders
- best candidates are single gene diseases caused by loss of function