Lecture 20

Question 1

molecular genetic techniques

Answer 1

localization of DNA/RNA sequences
analysis of genetic information
specific modification of genetic sequences
rearrangement (recombinant DNA)

Question 2

key innovations in molecular biology

Answer 2

recombinant DNA tools, polymerase chain reaction, DNA sequencing techniques, genome editing mechanisms

Question 3

recombinant DNA technology

Answer 3

finding the target gene in source genome, separate the target from the source, stabilize the target, identify and isolate host cells that received target DNA

Question 4

restriction enzymes

Answer 4

used to cut DNA at specific sequences, often resulting in sticky ends, sometimes blunt ends

Question 5

DNA ligase

Answer 5

allows recombing of DNA by binding two strands cut with the same restriction enzyme

Question 6

how to determine number of restriction sites

Answer 6

know the GC ratio of the target DNA, know the size of the genome, know number of bases in recognition site

Question 7

why are they called restriction enzymes

Answer 7

methylation of the host DNA protects it from being cut, allowing the foreign DNA to be cut by the restriction enzyme. prevents any damage to the host