Lecture 2 Exam Questions Flashcards

1
Q

Enzymes belong to which group of organic molecules?

A

Proteins

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2
Q

The substance that an enzyme works upon is called a:

A

Substrate

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3
Q

The thousands of different chemical reactions occurring in a cell at any moment are referred to as a cels:

A

Metabolism

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4
Q

Know where a non competitive inhibitor would bind on an enzyme

A
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5
Q

The process whereby cells completely break down glucose to COz and water for energy is:

A

cellular respiration

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6
Q

A molecule that undergoes reduction:

A

gains one or more electrons.

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7
Q

During glycolysis, the electrons that are removed from glucose are transferred to molecules ofi

A

NAD+

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8
Q

Which statement about glycolysis is FALSE?
Correct!
Glycolysis makes two molecules of pyruvic acid,
Glycolysis does not require oxygen
CO2 and H20 are products of glycolysis.
Glycolysis happens during fermentation

A

CO2 and H20 are products of glycolysis.

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9
Q

In prokaryotes, how many ATP molecules are produced from the breakdown of one glucose via aerobic respinaton

A

38

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10
Q

Put the following steps in order

l. Enzyme-substrate complex
ll. Substrate binds active site
lll. Enzyme free to interact with substrate
lV. Product release
V. Product is formed

A

lll, ll, l, V, lV

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11
Q

In yeast fermentation:

A

alcohol and carbon dioxide are given off

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12
Q

The NADH molecules formed during the Krebs cycle are

A

oxidized when electrons are passed to the electron transport chain

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13
Q

The production of dairy products such as cheese and yogurt or pickled foods requires which of the following

A

lactic acid fermentation

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14
Q

An organism that uses oxygen when present but can grow without oxygen is called an

A

facultative anacrobe.

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15
Q

Microbes that are introduced into a culture medium to initiate growth are called an

A

inoculum.

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16
Q

Your lab instructor hands you a culture and tells you that there are two different types of bacteria groning in it Pudonones and Staphylococcus. If you wanted to grow just Staphylococcus colonies, you would need to use an

A

selective medium

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17
Q

Even though the use of agar is sometimes attributed to Robert Koch, you know the real mastermind behind this great idea was

A

Fannie Hesse

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18
Q

During which growth phase are bacteria MOST susceptible to penicillin?

A

Log

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19
Q

A spectrophotometer can be used to measure:

20
Q

Aunt Melba brings her famous potato salad to the annual family Fourth of July picnic. She arives at 10.00 AM, and on one potato there is a small colony of 100 Salmonella bacteria. The edge of the potato salad is warm, and the cells divide every 20 minutes.
When you unknowingly scoop the entire colony onto your plate at 12.00 PM, how many salmonella are you infecting yourself with

21
Q

The phase of the bacterial growth curve in which newdy inoculated cells are adjusting to ther new envionnent life, (they are metabolizing but not actively dividing) is the:

22
Q

I find a gallon of milk at the back of my fridge and realize it is past its expiration date. 1 take a 1 ml. sample and perform a serial dilution, then inoculate a series of growth plates. After incubation, I count 36 colonies on the growth plate labeled 1:10,000 dilution
Thus, my original sample contained:

A

3.6 x 10^5 bacteria per mL

23
Q

To sterilize heat-sensitive solutions such as culture media, enzymes, and vaccines, one should use:

A

filtration

24
Q

In the assigned article, food and nutrition specialist Julie Garden-Robinson stated that tragedies similar to the Heirs delay decer part occurred around the same time in North Dakota because the food in the jars:

A

did not have enough acid.

25
The removal or destruction of ALL forms of microbial life Is called:
sterilization.
26
Beef jerky relies on which method of microbial control?
desiccation
27
To sterilize heat-sensitive solutions such as culture media, enzymes, and vaccines, one should use:
Filtration
28
An unopened carton of pasteurized milk spoils in the refrigerator. Testing of the milk reveals the presence of microngelent. The most likely explanation is
the microbes that survived pasturization were able to grow in the refrigerator
29
Water can be disinfected using all of the following. EXCEPT: peracetic acid. Ozone UV light Chlorine
Peracetic acid
30
The recommended optimum concentration of ethanol to use as a biocide is:
70%
31
Who introduced antisepsis into medical practice?
Semmelweis
32
Which of the following is NOT used to preserve food? benzoyl peroxide sodium nitrite sodium benzoate calcium propionate
benzoyl peroxide
33
One of the few liquid chemical disinfectants that can be considered a sterilizing agent and is also used for embalming is
glutaraldehyde.
34
Betadine is an iodine-containing solution that can be used to treat minor cuts and scrapes on the skin. Which of the folowing terms best describes what Betadine is used for?
antisepsis
35
Nosocomial infections are best avoided by:
washing your hands between patients.
36
Assume that you are responsible for decontaminating materials in a large hospital. How would you sterilize used disposable syringes?
ethylene oxide
37
A segment of DNA that codes for a functional product is a(n):
gene
38
In Chapter 8, we talked a lot about genes. Where would I find genes in a prokaryote?
the nucleoid region and plasmids
39
Flagella on a bacterial cell are an example of a:
phenotype
40
What carries the coded information for making specific proteins from DNA to ribosomes?
mRNA
41
In ________, a temporary copy of a gene is created. In ______, a protein is assembled.
transcription, translation
42
Which molecules recognize specific codons and transport amino acids?
tRNA
43
An mRNA molecule is 312 nucleotides in length. The first three nucleotides are AUG. The last three nucleotides are UAA, How nuny arico acids will be in the protein after translation?
103
44
The process of bacteria passing their genes to other microbes of the same generation is known as?
horizontal gene transfer
45
If the wild-type codon is UAU and a mutation changes it into UCU, this would be a ____ mutation
missense
46
The three steps in each PCR cycle are:
denaturation, annealing, extension
47
In transduction:
A virus is required for the transfer of genetic material.