Lecture 2 Flashcards
1
Q
function of major groove
A
*
2
Q
base pairs per turn of b dna
A
10
3
Q
relative dif between each form of dna
A
groove size, diameter, rise, pitch, handednes
4
Q
interactions stabilizing dsdna duplex
A
hydrophobic, van der waals
5
Q
chargaffs rule
A
[A]=[T] and [G]=[C]
6
Q
lactam vs lactim
A
*
7
Q
lactam/lactim dominates at pH 7
A
lactam (99.99%)
8
Q
if 0.01% lactim form, how many bases out of 3 B bp are in the lactim form
A
600,000
9
Q
increased/decreased ionic strength causes denaturation
A
decreased ionic strength
10
Q
how does pH effect denaturation
A
*
11
Q
left handed superhelix is positive/negative supercoiling
A
negative supercoiling
12
Q
predominant supercoil orientation
A
left handed superhelix (negative supercoil)
13
Q
define negative supercoiling
A
deficit of helical turns
14
Q
Lk, linking number
A
*
15
Q
specific linking difference
A
*
