Lecture 2 Flashcards
what kind of estrous cycles do dogs have
yearly nonseasonal diestrous
Time preceding mating in dogs
proestrus
during proestrus, the bitch is attractive to males but will not all what
mating
in dogs, how does the vulva appear in proestrus
swollen
In dogs, serosanguineous discharge is present during which estrous cycle
proestrus
In dogs, Estrogen causes vaginal epithelial cells to do what
cornify
what does cornified cells mean
largest cells in the smear
lack a nucleus
Time of sexual receptivity when bitch will allow mating
estrus
in dogs, vulvar swelling typically does what during estrus
decreases
In dogs, during estrus, bloody discharge changes to _____ color or may _______ throughout estrus
straw
continue
in dogs, what three things will a vaginal cytology show during estrus
100% Cornified vaginal epithelial cells
No White blood cells
Occasional Bacteria observed
the end of estrus
diestrus
time of pregnancy being established happens when
diestrus
in dogs, there is an abrupt decline in the percentage of _______ cells on day 1 of _______
cornified
diestrus
D1 =
day 1 of diestrus
in dogs, 4-8 days after LH surge (1-4 days before D1)
fertile period
in dogs, 5-6 days after LH surge (2-3 days before D1)
peak fertility
In dogs, Be sure that vaginal cytology is fully cornified during what time
mating schedule
Time when female is not undergoing any reproductive events. ‘Hormone quiescence’
Not “Not in Heat”
Anestrus
in dogs, three techniques for repro evaluations
Vaginal cultures for bacteria
Vaginal cytology
Hormone assays
in dogs, when are vaginal cultures usually done
when there are clinical issues indicating an issue
technique for vaginal cultures, to prevent contamination
guarded swab
in dogs, Used to analyze reproductive cycle
DOES NOT precisely predict ovulation
vaginal cytology
what is the technique for evaluating a vaginal cytology
Examine vaginal cells on prepared slide with microscope
Three steps for vaginal sample handling
- roll swab head onto a clean slide - don’t smear or grind onto slide
- air dry and stain
- examine under microscope
in dogs, what kind of vaginal epithelial cells will you see under the microscope
Noncornified cells
Cornified cells
in dogs, vaginal cytology cannot precisely predict what
ovulation
in dogs, what does 100% cornified cells mean on a vaginal cytology
bitch is in estrus
in dogs, when there is an abrupt decline of cornified cells on a vaginal cytology, what does this represent
End of estrus and start of Diestrus (D1)
in dogs, what can precisely predict ovulation
hormone assays
what hormone increases before ovulation - only in canines
progesterone
in dogs, With hormone assay, Initial serum progesterone increase correlates with
LH surge (release of oocytes from follicles)
in dogs, LH peak may occur from same day of, or up to _____ days after, full cornification
two
in dogs, can determine LH surge - The “Gold Standard”
Progesterone assay
in dogs, The initial rise of progesterone happens when
the same day as the LH surge
in dogs, when does ovulation occur
two days after the LH spike
in dogs, Commercially-available in-house kits assay what two hormones
LH or Progesterone
in dogs, how soon are in house assay results available
20 minutes
in dogs, LH can be tested in house but requires what
daily serum collection
in dogs, what will make a progesterone kit give false high reading
if the kits is too cold
in dogs, three reasons why a kit might give false low readings
Serum handled improperly
Hemolyzed sample
Lipemic sample
Four ways to determine pregnancy in the canine
Abdominal Palpation
Hormone assay
Ultrasound
Radiology
in dogs, abdominal palpitation for pregnancy detection can be performed during a ________ day window
7 to 10 day
in dogs, when can abdominal palpitation for pregnancy detection be use
21 days after D1
in dogs, when does the window close to use abdominal palpitation for pregnancy detection
D1 + 30 days
in dogs, What hormone do hormone assay tests use to determine pregnancy
relaxin
in dogs, When are hormone assay tests reliable for determining pregnancy
28 days post LH surge OR day 20 after D1
in dogs, when can ultrasound be used to establish pregnancy
D1+20 days - up to parturition
in dogs, What is the only reliable method to accurately determine number of pups, relative sizes, and position
Radiology
in dogs, when is radiology used to determine pregnancy
after D1+37 days
in dogs, how can you estimate gestation length
Use day of progesterone rise (LH spike) or D1
what is the range of gestation in dogs
55-70 days from breeding event
what is a more reliable predictor of the whelping date
D1 + 57-58 days
how long does stage 1 parturition last for dogs
6 - 12 hours (can go up to 36 hours)
two behaviors that bitches show in stage 1 parturition
restlessness
nesting
in dogs, what will the body temp drop to in stage 1 parturition
99 degress
how soon will 85% of bitch go to stage 2 parturition after the temp drop
within 24 hours