Lecture 19

Question 1

A gene library is:

Answer 1

a collection of DNA clones from an organism cloned into a vector

Question 2

A gene library is:

Answer 2

a collection of clones that represent the DNA or cDNA of an organism

Question 3

A small 1.5 mL tube can:

Answer 3

contain millions of plasmids or other vectors that contain the DNA

Question 4

A typical library will contain;

Answer 4

half a million clones

Question 5

clones derived from mRNA are called:

Answer 5

cDNA clones

Question 6

transformation is:

Answer 6

the introduction of new DNA into an organism

Question 7

transformation can be used:

Answer 7

for adding an additional gene or for gene replacement

Question 8

principle techniques used to transform higher organisms are:

Answer 8

Lipid vesicles
Eletroporation
Biolistic delivery
micro injection
virus infection

Question 9

he bacteria Agrobacterium tumefaciens has the
natural ability to

Answer 9

tranform, transfer foreign DNA to certain plant species with its Ti plasmid

Question 10

The bacteria Agrobacterium tumefaciens and a naturally
occurring plasmid of Agrobacterium have been modified
to introduce

Answer 10

engineered genes into plants

Question 11

transformation can be in:

Answer 11

somatic cells or in germline cells

Question 12

transformation of germline cells gives rise to :

Answer 12

transgenic gametes and the transformed genes are stably inherited generation after generation

Question 13

somatic cell transformation is often used in research to :

Answer 13

monitor the effect of a gene modification

Question 14

somatic cell transformation is ___ from generation to generation

Answer 14

not inherited

Question 15

Ashinti de Silva
was treated for
severe combined
immunodeficiency
disease (SCID)
by:

Answer 15

somatic
cell transformation.

Question 16

In many cases the integration of the transgene into the chromosome is :

Answer 16

random

Question 17

In many cases the integration of the transgene into the chromosome is random.The new gene can function, but:

Answer 17

it may not be in the location of the original gene in the organism

Question 18

__ is used for genome editing

Answer 18

CRISPR-Cas9

Question 19

CRISPR - Cas9 were discovered as :

Answer 19

sequence repeats in bacterial genomes that resembles virus sequences. They are part of a natural defense of bacterial cells to recognize and cut infecting virus genomes

Question 20

By introducing different plasmids into the cell, there can be different results (4):

Answer 20

a) Gene deletion
b) Gene replacement
c)Specific sequence change
d)increase expression of a specific gene, by combining an inactivated Cs9 with a transcriptional activatorI