lecture 19 Flashcards
diffusion
molecules move from one location to another by random, thermally driven motion
2 things that rate of diffusion depends on
- size (smaller= faster)
- solubility (hydrophobic= faster)
What is the membrane potential?
the voltage difference across the membrane (about 200mV)
transporters
only transport molecules/ions that fit into specific binding sites
channels
allow solutes through a channel, discriminate based on size and charge
3 characteristics of passive transport
diffusion of a small polar molecule
spontaneous movement of a solute down its electrochemical gradient
channels and transporters can do passive transport
moving a solute against its electrochemical gradient
active transport
active transport is performed by
pumps
requires energy
osmosis
movement of water down its concentration gradient from low to high solute concentration
aquaporins
channel proteins.
allow flow of water across plasma membrane.
mechanism: contractile vacuoles and rigid cell walls
glucose transporter is a
multipass transmembrane protein
does passive transport by changing its conformation
the flow of glucose ca go either way– depending on the concentration gradient
3 ways that pumps perform active transport
- coupled pumps
- ATP driven pumps
- light driven pumps
coupled pump
links uphill transport of one solute w the downhill transport of another
ATP driven pumps
hydrolyze ATP to drive uphill transport
light driven pumps
use energy derived from sunlight to drive uphill transport
Na+ pump– what goes in and out of cell?
pumps Na+ out of the cell and 2 K+ into the cell at the expense of ATP
Na+ pump concentration gradient
maintains a steep concentration gradient
high Na+ outside cell, high K+ inside cell
high Na+ outside acts like a dam storing energy
Ca2+ pump
pumps Ca2+ out of the cell at the expense of ATP
3 types of coupled pumps
uniport= only carries one solute
symport= moves two solutes in same direction
antiport= moves two solutes in opp directions
Glucose-Na+ symport
found on the apical side of gut cells
uses import of Na+ to drive the import of glucose
both Na+ and glucose must be present
plants, bacteria and fungi use — gradients
H+
–> increased [H+] in lysozome, gradient generated by H+ pumps will pump H+ out of the cell