LECTURE 19 Flashcards
what is the name of the process of transforming DNA to DNA ? what is the dependency and what will the polymerase?
replication.
DNA dependence and DNA polymerase
what is the name of the process of transforming DNA to RNA ? what is the dependency and what will the polymerase? is it reversible? what is the name of the reversible process? and what is the dependency and polymerase?
transcription
DNA dependence and RNA polymerase.
yes, it is reversible and the process name is revers transcription and it is RNA dependent and DNA polymerase
what is the name of the process of transforming RNA to polypeptides ? is it reversible ?
translation
not reversible
what happens if the process of DNA replication fail ?
the process of mitosis and meiosis come to the half
what are the complementary base pairs in DNA
A pairs with T, G pairs with C
how many hydrogen bonds are associated with the base pairs
Three hydrogen bonds pair G
with C, while two hydrogen bonds
pair A with T
how is the direction of DNA structure?
*Anti-parallel 5’ to 3’DNA strands
what is the most common form of DNA in living cells
right-handed double helix (BDNA): most common form in living cells
how many base pairs are available in each turn
*Ten base pairs per turn
how much is the distance between each helical turns in DNA and stacked bases
0.34 nm between stacked bases;
3.4 nm per helical turn
how does the semi-conservative copying mechanism work
- separate the parental strains
- synthesis the parental complementary strands , then we have two identical daughters molecules
what is the conservative replication
generating a new DNA without separating the DNA strands
what is the dispersive replication
in the dispersive replication we cut the DNA strands from the middle and then each separate part would synthesis the parental complementary strands
what technique is used to to separate double-stranded DNA (dsDNA) molecules of different densities
Meselson and Stahl used a technique called cesium chloride (CsCl) equilibrium density gradient centrifugation to separate double-stranded DNA (dsDNA) molecules of different densities
what is the separation is based on
-permits separation of dsDNA based on density
