Lecture 18 - Coding and Noncoding RNA Flashcards
What was the defect that caused hemophilia in the family of Tsar Nicholas?
L18 S3
Defect in a splice site that results in an nonfunctional factor IX.
What are the concepts of collinearity and noncollinearity?
How did an experiment demonstrate which of these was true in eukaryotes?
L18 S4-7
Collinearity:
-a continuous segment of DNA should code for a proportional amino acid sequence
Non-collinearity:
-a continuous segment of DNA does not code for a proportional amino acid sequence; there is noncoding DNA involved
When mRNA was introduced to DNA sequence that it was complementary with, loops would from in the DNA representing noncoding region, introns.
What flanks the protein coding region of mRNAs?
L18 S10
5’ and 3’ untranslated regions (UTRs)
What are the crucial sequences located at the 5’ and 3’ splice sites?
L18 S16
Consensus sequences
If a splice site were mutates so that splicing did not take place, what would the effect be on the protein encoded by the mRNA?
L18 S20
A. It would be shorter than normal.
B. It would be longer than normal.
C. It would be the same length but would have different amino acids.
A. It would be shorter than normal.
There would almost certainly be a random stop codon located within ~20 codons of the faulty splice site that will result in early termination of the protein.
What is guide RNA and what does it do?
L18 S26
RNA that is partially complementary to preedited mRNA and binds it. After binding, the mRNA is cleaved at the sites that aren’t complementary and nucleotides are added.
Where do miRNAs bind?
L18 S28
3’ UTR
