Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Genetics > lecture 16 > Flashcards

lecture 16 Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

can dsRNAs be synthesized in the lab?

A

yes, you just need the sequence of the target mRNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

what is eRNAi

A

embryonic RNAi

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what is mRNAi

A

maternal RNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

what are the main methods of reverse genetics?

A

TALEN, CRISPR, and Zinc finger nuccleases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

what causes mutation by double stranded breaks?

A

non-homologous end joining (NHEJ)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

zinc-finger nucleases

A

artificial restriction enzymes made by the fusion of a zinc-finger transcription factor domain and a DNA- cleavage domain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

is a DNA- cleavage domain specific or non-specific?

A

non-specific

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

what is the ONLY way ZFNs can be used?

A

in pairs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

TALENS

A

transcription activator-like effector nuclease

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

how are TALENS made?

A

the fusion of the DNA binding domain of a bacterial transcription factor (TAL) to a DNA cleavage domain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

is the DNA cleavage domain sequence specific ?

A

no, but researchers can engineer it to be

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

how do TALs work?

A

they recognize DNA through 34 AA repeat sequence, but the 12th and 13th position are hypervariable, meaning there is no code

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

how many TALENs do you need for a double stranded break?

A

2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

what does CRISPR stand for?

A

Clustered Regularly Interspaced Short Palindromic Repeats

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

what is CRISPR?

A

an adaptive immunity system in bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

what is CRISPR derived from?

A

bacteriophages that had previously infected the bacteria

17
Q

what is CRISPR used for?

A

to detect and destroy DNA from similar viruses during subsequent infections

18
Q

what happens to the piece of viral DNA that was proteins ut off?

A

they are integrated into the bacterial genome in between specific spacer regions

19
Q

why does the bacteria store viral DNA?

A

the bacteria can remember the virus and will be able to counter-attack it if it invades again

20
Q

what are repeat sequences?

A

they separate the pacer/viral sequences

21
Q
A

Genetics (7 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions