Lecture 15

Question 1

What allows for different compartments to be created inside a nucleus?

Answer 1

Different chromatin modifications allow for sorting in different compartments

Question 2

What happens in DNA looping?

Answer 2

Cohesions protein has a motor that pushes the DNA to make a loop but it stops when the base of the chromosomes reach CTCF where there are CTCF binding sites.
*CTCF defines where cohesions is going to stop

Question 3

Describe the core promoter and why it is so important

Answer 3

The core promoter has TATA box (not all promoters do) which helps to initiate transcription
- The TATA Binding protein or TBP recruits RNA Polymerase II
- Once bounds, RNAP II sits and waits, ready to be activated by TFs that are bound to enhancers.

• The core promoter tends to be located in nucleosome free regions
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