lecture 13- PDH

Question 1

cAMP

Answer 1

a signaling molecule that regulates glycolosis

Question 2

pyruvate dehydrogenase

Answer 2

takes pyruvate and converts it to Acetyl CoA, also produces NADH and CO2 (feeds into TCA cycle).

Question 3

co enzyme A

Answer 3

composed of adenosine phosphate, vitamin B5, and a reactive thiol group. it links with acetyl to create acetyl CoA

Question 4

pyruvate dehydrogenase complex

Answer 4

E1 subunit: bound to TPP which reacts with pyruvate which creates hydroxyethyl TPP
E2: dihydrolipoyl transacetylase, generates acteyl CoA
E3: contains FAD cofactor which regenerates FAD and NADH

Question 5

pyruvate decarboxylase

Answer 5

turns pyruvate into alcohol/yeast

Question 6

1. Decarboxylation in pyruvate dehydrogenase

Answer 6

TPP carbanion and pyruvate form hydroxyethyl TPP

Question 7

2. Oxidation in pyruvate dehydrogenase

Answer 7

from E1 to E2 subuint

Question 8

3. Transfer in pyruvate dehydrogenase

Answer 8

E2 to E3 handoff, needs FAD

Question 9

4. Regeneration

Answer 9

E3 regenerates oxidized lipoic acid and generates NADH