Lecture 11 - Molecular Genetic Testing Flashcards
Which type of test isolates RNA in order to identify and quantify specific mRNAs?
Northern blot
Which type of test digests test DNA using gel electrophoresis and hybridization to a probe?
Southern blot
Which test amplifies DNA using both primers/templates and DNA polymerase?
PCR
Which test isolates specific DNA regions and detects mutations?
PCR
Which test is an analysis of the protein content of a cell?
Proteomics
Which test identifies expression levels of proteins in normal vs. disease tissues?
Proteomics
What enzyme produces DNA fragments that can be easily joined together?
Restriction nucleases
What test finds or detects the DNA content of an organism and allows for gene expression profiling?
DNA Microarray hybridization
Northern blot hybridization is used to evaluate the _________ ______ _________ of a gene.
gross expression patterns
Which test is used to examine PROTEIN (specifically the molecular weight of a protein and its abundance in a specific tissue)?
Western blot
Which test identifies if a genomic locus is modified (i.e. insertions/deletions)?
Southern blot
What do restriction nucleases produce?
DNA fragments (with the same cohesive ends) that can be easily joined together
What are the 3 major steps involved in PCR?
- Denature strands (heat)
- Anneal strands (cool)
- Synthesize protein (incubate with DNA pol)
_____ is an endonuclease that associates with a specific CRISPR RNA sequence.
Cas9
Introduction of:
a) a mutant of the target gene,
b) the CRISPR-Cas9 sequence
… into a cell, promotes what?
homologous recombination at the site of ds DNA cleavage - such that the mutated gene replaces the original normal copy of the gene
