Lecture 11: CRISPR & Genetic Maniplation II

Question 1

Examples where CRISPR knock-ins could be used:

Answer 1

•Site-directed mutagenesis = changing an amino acid at a specific position

•‘Repairing’ a small deletion in a gene sequence

•Integrating a tag into a gene / region of genome

Question 2

kinases:

Answer 2

an enzyme that phosphorylates target proteins

Question 3

different types of tags:

Answer 3

• affinity tag
• fluorescent tag (GFP)
• degron tag

Question 4

CRISPR application in agriculture:

Answer 4

•Increase crop yield

•Enhance nutrient content

•Disease-resistance

•Drought-resistance

•Streamlined breeding

Question 5

CRISPR & global warming:

Answer 5

• altering the gut-microbiome of cattle so that the beneficial digestive bacteria create less methane: massive reduction in global greenhouse gas emissions

Question 6

problem with CRISPR:

Answer 6

guideRNA will be homologous to several regions of the genome - so may cut somewhere other than the desired locus: this is a major concern over using CRISPR therapeutically