Lecture 10: mechanisms of catalysis Flashcards

1
Q

transition state = (higher/ lower) energy state

A

higher energy state

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2
Q

stabilizing the t state will (inc/ dec) rate of the rxn

A

increase rate

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3
Q

2 ways to stabilize t state

A
  • protonate carbonyl oxygen making carbonyl carbon more electrophilic [acid catalysis]
  • deprotonate H2O making O more nucleophilic [base catalysis]
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4
Q

metal ion catalyst

A

electrostatic stabilization of a charged group in the t state

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5
Q

when Vo is higher at lower pH, His is

A

protonated [acid catalysis]

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6
Q

when Vo is lower at lower pH, His is

A

deprotonated [base catalysis]

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7
Q

the pH-rate profile of an enzyme-catalyzed reaction will affect ionization states of

A

all species [enzyme and substrate] in the rate limiting t-state

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8
Q

chymotrypsin

A

endoprotease that functions during intestinal digestions; cleaves both proteins and esters

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9
Q

the intermediate of chymotrypsin-catalyzed hydrolysis of an ester is

A

between burst and steady phase; not active

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