Lecture 10 (2/10)

Question 1

When patient HM had a bilateral resection of the hippocampus + surrounding structures, what happened

Answer 1

• Loss of declarative memory
• Couldn’t convert immediate memory into long term memory
• Intact intellectual + perceptual fxn

Question 2

Hebb’s rule

Answer 2

• Hypothesis for lasting memory

- Repeated firing from cell A –> cell B leads to increase in path efficacy

Question 3

Most studied connection pathway model system in hippocampus

Answer 3

Scaffer collateral pathway (CA3-CA1)

Question 4

Principal exictatory neurons in CA1 are _________ w/ ____________.

Answer 4

Principal excitatory neurons in CA1 are pyramidal neurons w/ spiny dendrites.

Question 5

induction phase (LTP)

Answer 5

High frequency stimulation indcuces specific cellular and synaptic signaling

Question 6

Expression phase (LTP)

Answer 6

Long-term change of synaptic strength that takes place @ synapse

Question 7

Which excites which in Schaffer collateral? (CA1 + CA3)

Answer 7

CA3 excites CA1

Question 8

CV^(-2) all equations

Answer 8

M^2/(sigma)^2 = np(1-p)

Question 9

What does CV^-2 reflect?

Answer 9

Presynaptic conditions! Takes into account p, n, and a, and the subsequent post synaptic response

Question 10

What happens to CV^-2 after LTP induction?

Answer 10

CV^-2 increases?

Question 11

What happens to synaptic failure rate after LTP induction?

Answer 11

Synaptic failure rate decreases

Question 12

What presynaptic condition is decreased synaptic failure linked to?

Answer 12

P! The probability that a quantum will be released

Question 13

I/V relationship for GluA2-containing AMPArs

Answer 13

Linear

Question 14

GluA2 containing AMPArs

1. have _______ I/V relationship
2. impermeable to ______
3. __________ rise and decay

Answer 14

GluA2 containing AMPArs

1. have linear I/V relationship
2. impermeable to calcium
3. fast rise and decay

Question 15

NMDA receptor channels

1. voltage dependent _________
2. _______ to calcium
3. __________ rise and decay

Answer 15

NMDA receptor channels

1. voltage dependent magnesium blockade
2. permeable to calcium
3. slower rise and decay

Question 16

Does APV impact the early/peak I or the late current? How?

Answer 16

The late current!

More prominent high late current @ high (more positive) mV values without APV

–> late current from NMDA receptors, APV blocks NMDA receptors

Question 17

What is APV?

Answer 17

An NMDAr antagonist

Question 18

How are silent synapses unsilenced during LTP?

Answer 18

More AMPArs are inserted into the postsynaptic membrane

Question 19

GluA2 containing AMPArs

1) _________ I/V
2) __________ to Na
3) __________ to K
4) __________ to Ca
5) _________ rise and decay

Answer 19

GluA2 containing AMPArs

1) linear I/V
2) permable to Na
3) permeable to K
4) impermeable to Ca
5) faster rise and deca

Question 20

GluA2 lacking AMPArs

1) _________ I/V
2) __________ to Na
3) __________ to K
4) __________ to Ca
5) _________ rise and decay

Answer 20

GluA2 lacking AMPArs

1) inward recitfying I/V
2) permeable to Na
3) permeable to K
4) permeable to Ca
5) slower rise and decay

Question 21

Which receptors should be fused with GFP to create an AMPAr electrophysiological tag?

Answer 21

GluA1 (GluR1)

Question 22

What is an electrophysiological tag for GluA2 lacking AMPArs?

Answer 22

Inward rectification in the I/V graph

Question 23

AMPAr currents show ______ rectification in GluR1-GFP expressing neurons after LTP induction

Answer 23

AMPAr currents show increased rectification in GluR1 expressing neurons after LTP induction

Question 24

LTP induction –> _____ GFP expression from GluA1-GFP

Answer 24

LTP induction –> enhanced GFP expression from GluA1-GFP