Lecture 1 Flashcards
1
Q
How do origins of replication work?
A
- ORC binds to ori alongside helper proteins
- Mcm helicases are loaded unto DNA net to ORC (pre-replicative complex)
- Specialised kinases phosphorylate mcm and ORC
- Helicases are activates and ORC is displaced, DNA pol and other replicative proteins are recruited.
- ORC rebinds and DNA synthesis occurs
2
Q
Why is telemore repication a problem
A
DNA synthesis always goes 5’ to 3’ but DNA is read 3’ to 5’
Rear primer near end of chromosome on lagging strand can’t be replaced with DNA since DNA polymerase must bind to a primer sequence.
3
Q
How does telomerase work?
A
Teloemrase attach a little stretch of DNA at the 3’ end of the DNA which allows DNA ligases to bind to it and fill the gaps.
4
Q
Where is the alpha cluster of Hg found?
Beta cluster?
A
Chromosome 16
Chromosome 11
5
Q
How can eukaryotic genomes be characterised using reassociation genetics?
A
A measure to predict the number of genes per genome. and non repeitive/repetitive DNA
