Lecture 1 Flashcards
Which sample is used for biochemical analysis?
Usually serum is preferred.
In horses, heparinized plasma is used because horse blood takes a long time to clot
What is the difference between serum and plasma?
Serum does not have clotting factors which plasma does
What are some things that can affect spectrophotometry?
Errors in sample collection Lipemia Hemolysis and oxyglobin Icterus Certain drugs and tests
What are three ways to maintain good quality control?
Use reference labs
Have competent and well-trained staff
Use good point of care testing
What are some advantages to point of care testing?
Improved turn around time
Potential for improved patient care/monitoring
Smaller sample volumes and smaller amounts of reagents
Its available outside reference labs normal hours
Available in rural areas
Can save costs if results resort in shorter hospital stays
What are some disadvantages of POCT?
Poor quality assurance
Poor quality of analysis due to operator erros
Poor record keeping
Poor cost capture and possible lost revenue
Different reference intervals than lab so can’t compare results
Expensive
What chemistry values are in a hepatic profile?
BUN Albumin Glucose ALT ALP GGT T Bili Cholesterol Urine bilirubin
What chemistry analytes are in a renal profile?
BUN Creatinine BUN: Creat Urine SP K P Ca Na Cl Albumin
What chemistry analytes are in a GI profile?
BUN: Creat TP Albumin Globulins Cholesterol Ca Na K Cl TCO2
What factors may influence your interpretation of the lab results?
Age, species, breed, hydration status, concurrent diseases, prior drug administration
What to consider when looking into a POCT machine?
What are the hospitals need?
What patient groups require testing?
Will POCT enable more rapid diagnosis/ treatment?
How does the cost compare?
How does the quality compare?
Can records be accurately kept with POCT machine? Can it work with the existing hospital interface?
Is there appropriate space?
Who will run the machine? How will they be trained?
What to consider when purchasing a POCT machine?
Direct and indirect costs
Internal QC functions
Cost/length of maintenance/ service contracts
Time investments
Operator training and CE
Troubleshooting
Turnaround time
Type of testing
How you will maintain inventory
What sample the machine requires
Customer support
Health and safety
How long should QC data be archived?
Two years- required by law
