Lecture 1/27 Flashcards
Is a somatic cell diploid or haploid?
Diploid, 2n
Is a germ cell diploid or haploid?
become haploid, n
What are 3 things the proks don’t have?
nucleus, ER, mitochondria
how much of each nucleotide is there in relation to each other?
A is approx equal to T and G is approx equal to C
heated S + R =
virulent
heated S =
avirulent
S+R =
virulent
Which strain of strep was virulent?
S
mutated S to R =
avirulent
What did the strep experiments tell us?
DNA is the transforming principle
What element can be used to label protein?
S35
What element can be used to label DNA?
P32
What is the life cycle of a bacteriophage?
- attach to host bacteria
- inject its genes into host cell
- phage DNA replicates; new phage proteins are made
- phage particles assemble
- cell bursts, releasing new phage
phosphates of deoxyribose attach to which carbon?
5
which carbon does a base attach to?
1
which carbon gets the hydroxyl group?
3
why do you need a triphosphate for DNA replication?
you need the energy
which end of the DNA strands are nucleotides added to? why?
3’ end because they need the hydroxyl group
which end of the DNA strand has a phosphate group?
5’
where on the DNA helix is the sugar-phosphate?
the outside, it’s the backbone
where are the base pairs on the dna helix?
the middle/inside
what are the 2 chains of DNA held together by?
H bonds
which is harder to split apart? A-T or G-C?
A-T is 2 H bonds and G-C is 3, so G-C is harder
