Lecture 1

Question 1

Name an Erythroid cell

Answer 1

Red blood cell

Question 2

Name an Hematopoiesis cell

Answer 2

B-cells, macrophages, T-cells

Question 3

Name a Myogenesis cell

Answer 3

Cardiomyocyte, Smooth muscle cell

Question 4

Name a Neural cell

Answer 4

Astrocyte, Microglia, Oligodendrocyte, Photoreceptor

Question 5

Name a Signalling cell

Answer 5

Beta cell

Question 6

Name a Structural cell

Answer 6

Chondrocyte, Osteoblast

Question 7

What cyclin allows entry into G1 phase

Answer 7

Cyclin D

Question 8

What cyclin allows entry into S phase

Answer 8

Cyclin E

Question 9

What cyclin allows entry into G2 phase

Answer 9

Cyclin A

Question 10

What cyclin allows entry into mitotic phase

Answer 10

Cyclin B (concentrations fall rapidly as anaphase commences)

Question 11

How do cyclin dependant kinases (CDKs) become activated

Answer 11

CDKs must bind to a cyclin protein and be in a particular phosphorylation state.

Question 12

What is the nuclear export signal (NES)

Answer 12

NES helps to shuttle cyclin B between the nucleus and the cytoplasm. It is rich in Leucine

Question 13

What are the steps of SDM

Answer 13

1) Oligonucleotide primers induce mutation in plasmid
2) PCR
3) Digest the DNA using Dpn1 enzyme to leave only mutated
4) Competent E. coli cells are transformed when they take up mutated plasmid