Lecture 1 Flashcards
Basic immunology, flow cytometry, RNA-seq, intravital microscopy
what do DAMPs and PAMPs trigger? (5)
- cytokine/chemokine secretion
- immune cell recruitment
- inflammation
- adaptive immunity
- tissue repair
what can the complement system lead to? (4)
- attack pathogen directly
- recruit more cells to help clear infection
- cause inflammation
- can cause coagulation
what is diapedesis? what type of cell typically undergoes diapedesis?
immune cells moving out of blood vessel into tissue with inflammation
mainly neutrophils
origins of tissue-resident cells?
can be myeloid or lymphoid and implanted at birth
what is the purpose of flow cytometry?
to detect chemical and physical characteristics/phenotypes of cells or particles
how does flow cytometry work?
use cell suspension from tissue, blood, bone marrow, or tumour
stain with fluorescently-tagged Ab that can bind antigens in the cell –> these markers let you figure out what’s happening in the cell and the type of cell
lasers hit the sample and Ab will emit diff light based on fluorophore –> find diff cell populations with diff markers
what is gating?
focus on one population and look at other markers –> i.e. look at diff populations within 1 population
what is forward scatter?
tells you the size of the cell
what is side scatter?
tells you the granularity of the cell
do forward and side scatters rely on the Ab markers?
no
what is the transcriptome?
all RNA transcripts
what is BULK RNA-seq?
looking at the tissue, can see what genes are upregulated/downregulated at specific times
(ex. compare genes in tumour vs normal tissue)
what is single cell RNA sequencing?
looking at the transcriptomics of a single cell –> gives mRNA from each cell and can identify phenotype and types of diff cells involved
why is intravital microscopy helpful?
immune cells are dynamic and constantly moving, so this lets us look at their movement
