Lecture 1

Question 1

monoclonal antibodies (mAB)

Answer 1

used as therapeutic agents
rapid growing group of pharmaceutical molecules

Question 2

monoclonal

Answer 2

produced from single B-lymphocyte clone treats cancer, inflammatory, infectious disease

Question 3

OKT3

Answer 3

first mAB that targets CD3 for T cell proliferation that causes T cells to deplete and reduce immune response

Question 4

antibody structure

Answer 4

top variable region where antigen binds and bottom is constant region

Question 5

Immunoglobins (IgG)

Answer 5

Variable L+H chains are antigen binding sites, cysteine creates disulfide bonds, loops are protease sensitive
loops on antibody allow for flexibility & Fab + Fc orientation varies
ScFv is used for antigen recognition

Question 6

2 fragments of IgG

Answer 6

Fab and Fc
Fab: contains variable region and is responsible for new drug development since drugs bind to variable region

Question 7

IgG functions

Answer 7

functions of proteins depends on binding other molecules so recognition is highly specific (complementarity)

Question 8

IgG protein binding and molecular recognition

Answer 8

protein function depends on binding because it induces a covalent interaction like an induced fit - binding causes chemical/spacial change to enhance specificity and complementarity
hypervariable region - exact complementary match between antigen and loop, induced fit, high specificity, high affinity

Question 9

Therapeutic Antibody Mechanism

Answer 9

targeting extracellular ligand receptor interactions causes antigen binding. IgA monoclonal antibody blocks SAR-COVs protein interaction with ACE2